PMID:12652910

Citation	Kang, L, Zhu, H, Cheng, Q, Zhou, W, Sun, L, Cai, L, Ma, X, Chen, C, Zhao, S and Li, C (2002) Cloning and characterization of a gene encoding glutathione-regulated potassium-efflux system protein KefKL from the endosymbiont Wolbachia. DNA Seq. 13:375-81
Abstract	The maternally inherited intracellular symbiont Wolbachia is well known for inducing a variety of reproductive and developmental abnormalities in the diverse arthropod hosts it infects. It has been implicated in causing cytoplasmic incompatibility (CI), parthenogenesis, feminization of genetic males and male killing in different hosts. However, the molecular mechanisms by which this fastidious bacterium causes these abnormalities have not yet been determined. In our study, representational difference analysis (RDA) was used to analyze the genomic difference between different Wolbachia strains. A gene encoding glutathione-regulated potassium-efflux system protein KefKL from Wolbachia in Drosophila simulans Riverside (w Ri) was isolated. The homologous genes from Wolbachia in Drosophila melanogaster yw67c23 (wMel) and Wolbachia in Drosophila melanogaster CantonS (wMelCS) were also cloned and sequenced. Sequence analysis showed that these deduced amino acid sequences contained two important motifs: Na+/H+ antiportor and NAD binding domain, which shared conserved sequences among different strains. Considering the crucial function of KefKL for ionic homeostasis, this gene might play an important role in Wolbachia physiology. Further study indicated that there was no homologue detected from Wolbachia in Drosophila simulans DSW/Mau (wMa) and Wolbachia in Drosophila simulans Noumea (wNo). Whether Wolbachia contained KefKL (or the homologous gene) was consistent with the phylogenetic studies using wsp sequences, which showed that wMa and wNo were grouped into one branch, while w Ri, wMel and wMelCS were more closely related.
Links	PubMed
Keywords	Amino Acid Sequence; Base Sequence; Glutathione/metabolism; Molecular Sequence Data; Potassium-Hydrogen Antiporters/genetics; Potassium-Hydrogen Antiporters/metabolism; Sequence Analysis, DNA; Wolbachia/genetics; Wolbachia/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9RICK:Q93LF0		GO:0006813: potassium ion transport	ECO:0000247:	UniProtKB:P45522	P		Figure 2 shows the alignment of e.coli K12 KefB to wolbachia KefKL gene. E.coli K12 KefB is annotated as potassium ion transport inferred from genetic interaction reported in the PMID:3301813.	complete CACAO 8135
9RICK:Q93LF0	involved_in	GO:0006813: potassium ion transport	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P45522	P		Seeded From UniProt	complete

References

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