PMID:7689140

Citation	Holden, PR, Brookfield, JF and Jones, P (1993) Cloning and characterization of an ftsZ homologue from a bacterial symbiont of Drosophila melanogaster. Mol. Gen. Genet. 240:213-20
Abstract	A 1194 bp open reading frame that codes for a 398 amino acid peptide was cloned from a lambda gt11 library of Drosophila melanogaster genomic DNA. The predicted peptide sequence is very similar to three previously characterized protein sequences that are encoded by the ftsZ genes in Escherichia coli, Bacillus subtilis and Rhizobium meliloti. The FtsZ protein has a major role in the initiation of cell division in prokaryotic cells. Using a tetracycline treatment that eradicates bacterial parasites from insects, the ftsZ homologue has been found to be derived from a bacterium that lives within the D. melanogaster strain. However, polymerase chain reaction (PCR) amplification of the gene from treated embryos suggests that it is not derived from a gut bacterium. Nevertheless, by amplifying and characterizing part of the 16S rRNA from this bacterium we have been able to demonstrate that it is a member of the genus Wolbachia, a parasitic organism that infects, and disturbs the sexual cycle of various strains of Drosophila simulans. We suggest that this ftsZ homologue is implicated in the cell division of Wolbachia, an organism that fails to grow outside the host organism. Sequence and alignment analysis of this ftsZ homologue show the presence of a potential GTP-binding motif indicating that it may function as a GTPase. The consequences of this function particularly with respect to its role in cell division are discussed.
Links	PubMed
Keywords	Amino Acid Sequence; Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Blotting, Southern; Cloning, Molecular; Cytoskeletal Proteins; DNA, Bacterial; Drosophila melanogaster/microbiology; GTP-Binding Proteins/genetics; GTP-Binding Proteins/metabolism; Larva; Molecular Sequence Data; Polymerase Chain Reaction; RNA, Bacterial/genetics; RNA, Ribosomal, 16S/genetics; Rickettsieae/genetics; Rickettsieae/metabolism; Rickettsieae/physiology; Sequence Homology, Amino Acid; Symbiosis; Tetracycline/pharmacology
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
WOLSP:FTSZ		GO:0051301: cell division	ECO:0000247:	UniProtKB:P0A9A6	P		Fig 3. In E.coli K12, ftsZ functions in cell division inferred from mutant phenotype PubMed 2045370. Source: EcoliWiki	complete CACAO 8234
WOLSP:FTSZ		GO:0005737: cytoplasm	ECO:0000247:	UniProtKB:P0A9A6	C		Fig 3. In E.coli K12, ftsZ is located in cytoplasm inferred from direct assay PubMed 1943703. Source: EcoliWiki	complete CACAO 8235
WOLSP:FTSZ		GO:0005525: GTP binding	ECO:0000247:	UniProtKB:P0A9A6	F		Fig 3. In E.coli K12, ftsZ has GTP binding activity inferred from direct assay PMID:1528267 PMID:1528268 . Source: EcoliWiki	complete CACAO 8236
WOLSP:FTSZ		GO:0051258: protein polymerization	ECO:0000247:	UniProtKB:P0A9A6	P		Fig 3. In E.coli K12, ftsZ functions in protein polymerization inferred from direct assay PubMed 21321206 and PubMed 8917533 Source: EcoliWiki	complete CACAO 8237
WOLSP:FTSZ	enables	GO:0005525: GTP binding	ECO:0000247: sequence alignment evidence used in manual assertion	UniProtKB:P0A9A6	F		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.