Category:Team The Bioneers

Figure 6a shows that the authors were directly measuring the glutamine synthesis by the reaction L-glutamate + ATP + NH(4)(+) = L-glutamine + ADP + 2 H(+) + phosphate., using the transferase assay (see methods).

Figure 5 shows the results of a direct assay of Mat1, Mat2 and Mat3 showing that they all utilized malonyl CoA as the thioester donor, and converted all the tested 7-O-glycosidic isoflav- ones and flavanones into a corresponding products. The authors went further and said that "Collision-induced dissoci- ation (CID) fragmentation of the product suggested that malonylation occurred at the 7-O-glycoside"

Investigators report failure of APTase activity in npt1-null yeast mutants.

Figure 2 displays the histone deacetylation (HDA) in mutant yeast strains, characterizing the importance of Hst2p gene product to NAD(+)-dependent HDA activity.

Figure 3 shows the direct measurement of the degradation of oligoribonucleotides upon exposure to NrnC protein. Specifically, the 3'-5' activity is known because the oligoribonucleotices were fluorescently labeled on their 5' ends to know that the 3' end is degraded before the 5' end

On page 777, the authors state that they performed direct assays where MgsA protein is incubated with its substrate, DHAP and methylglyoxal is produced. The methylglyoxal is said to have been measured spectrophotometrically.

Table 2 shows that Netrin-4 down-regulation in zebrafish leads to blood vessel formation defects and the authors additionally noted that only blood vessel formation was altered, not blood circulation, heartbeat or somite formation.

Figure 4 characterizes the association of Drosophila eIF3 to the 40S ribosomal subunit during normal and active translation.

table 2 shows that the knockdown of DNG701 causes DNA Hypermethylation of Tos17 at 5m-cytosine in rice and table 3 shows that overexpression of DNG701 Leads to DNA hypomethylation in rice

Table 1 shows that the researchers directly measured the conversion of Glc-1-P to Glc-6-P in various Oxidative situations amongst PGMC1 mutants.

Null mutant phenotypes demonstrated diminished proteolysis of SecY24

Knockout phenotypes demonstrated diminished proteolytic activity.