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PMID:22262096
| Citation |
Liu, MF, Cescau, S, Mechold, U, Wang, J, Cohen, D, Danchin, A, Boulouis, HJ and Biville, F (2012) Identification of a novel nanoRNase in Bartonella. Microbiology (Reading, Engl.) 158:886-95 |
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| Abstract |
In Escherichia coli, only one essential oligoribonuclease (Orn) can degrade oligoribonucleotides of five residues and shorter in length (nanoRNA). In Bacillus subtilis, NrnA and NrnB, which do not show any sequence similarity to Orn, have been identified as functional analogues of Orn. Sequence comparisons did not identify orn, nrnA or nrnB homologues in the genomes of the Chlamydia/Cyanobacteria and Alphaproteobacteria family members. Screening a genomic library from Bartonella birtlesii, a member of the Alphaproteobacteria, for genes that can complement a conditional orn mutant in E. coli, we identified BA0969 (NrnC) as a functional analogue of Orn. NrnC is highly conserved (more than 80 % identity) in the Bartonella genomes sequenced to date. Biochemical characterization showed that this protein exhibits oligo RNA degradation activity (nanoRNase activity). Like Orn from E. coli, NrnC is inhibited by micromolar amounts of 3'-phosphoadenosine 5'-phosphate in vitro. NrnC homologues are widely present in genomes of Alphaproteobacteria. Knock down of nrnC decreases the growth ability of Bartonella henselae, demonstrating the importance of nanoRNase activity in this bacterium. |
| Links |
PubMed Online version:10.1099/mic.0.054619-0 |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bartonella/enzymology; Bartonella/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Exoribonucleases/genetics; Exoribonucleases/metabolism; Gene Knockdown Techniques; Genetic Complementation Test; Genomic Library; Molecular Sequence Data; RNA Stability; RNA, Bacterial/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008408: 3'-5' exonuclease activity |
ECO:0000314: |
F |
Figure 3 shows the direct measurement of the degradation of oligoribonucleotides upon exposure to NrnC protein. Specifically, the 3'-5' activity is known because the oligoribonucleotices were fluorescently labeled on their 5' ends to know that the 3' end is degraded before the 5' end |
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