User:Hufnag30

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ORYSJ:B8YIE8	2012-04-12 20:36:41 CDT	GO:0051747 cytosine C-5 DNA demethylase activity (F)	PMID:21896764	ECO:0000315 mutant phenotype evidence used in manual assertion	table 2 shows that the knockdown of DNG701 causes DNA Hypermethylation of Tos17 at 5m-cytosine in rice and table 3 shows that overexpression of DNG701 Leads to DNA hypomethylation in rice	challenge
updatedbyinstructor	DANRE:Q32SK8	2012-04-14 15:22:37 CDT	GO:0001944 vasculature development (P)	PMID:22179604	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 2 shows that Netrin-4 down-regulation in zebrafish leads to blood vessel formation defects and the authors additionally noted that only blood vessel formation was altered, not blood circulation, heartbeat or somite formation.	challenge
acceptable	BACSU:MGSA	2012-04-14 15:44:46 CDT	GO:0008929 methylglyoxal synthase activity (F)	PMID:21992469	ECO:0000314 direct assay evidence used in manual assertion	On page 777, the authors state that they performed direct assays where MgsA protein is incubated with its substrate, DHAP and methylglyoxal is produced. The methylglyoxal is said to have been measured spectrophotometrically.	challenge
requireschanges	9RHIZ:G4VUY7	2012-04-14 16:38:00 CDT	GO:0008408 3'-5' exonuclease activity (F)	PMID:22262096	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 shows the direct measurement of the degradation of oligoribonucleotides upon exposure to NrnC protein. Specifically, the 3'-5' activity is known because the oligoribonucleotices were fluorescently labeled on their 5' ends to know that the 3' end is degraded before the 5' end	challenge
acceptable	MEDTR:B4Y0U0	2012-04-15 10:11:22 CDT	GO:0016420 malonyltransferase activity (F)	PMID:18419782	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 shows the results of a direct assay of Mat1, Mat2 and Mat3 showing that they all utilized malonyl CoA as the thioester donor, and converted all the tested 7-O-glycosidic isoflav- ones and flavanones into a corresponding products. The authors went further and said that "Collision-induced dissoci- ation (CID) fragmentation of the product suggested that malonylation occurred at the 7-O-glycoside"	challenge
acceptable	MEDTR:Q84UC1	2012-04-15 10:52:10 CDT	GO:0004356 glutamate-ammonia ligase activity (F)	PMID:16136328	ECO:0000314 direct assay evidence used in manual assertion	Figure 6a shows that the authors were directly measuring the glutamine synthesis by the reaction L-glutamate + ATP + NH(4)(+) = L-glutamine + ADP + 2 H(+) + phosphate., using the transferase assay (see methods).	challenge

acceptable:4
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Hufnag30

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Hufnag30

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	MAIZE:PGMC1	2012-04-01 07:51:44 CDT	GO:0004614 phosphoglucomutase activity (F)	PMID:9662542	ECO:0000314 direct assay evidence used in manual assertion	Table 1 shows that the researchers directly measured the conversion of Glc-1-P to Glc-6-P in various Oxidative situations amongst PGMC1 mutants.	challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Hufnag30

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Hufnag30