Category:Team T7

TABLE 3. Molecular weights and copy numbers of baseplate constituents

Authors use missense mutations and sequence rIIA gene of bacteriophage T4.

Authors sequence tail fiber genes in assay of T4.

Authors align full genome with NCBI T4 genome and this protein is identified. Authors deposit new genome in Genbank.

Table 2

Fig 7

Fig 3

Fig 2 "Self-ADP-ribosylation of ModA and ModB was also observed in the reactions"

Figs 7,8,9.

As shown in Figure 1, the L-shaped tail fibers in T5 increase the absorption rate by reversibly binding to LPS on the host. The figure shows mutant hosts with more available O-antigens increase the absorption rate of T5 phage into the host.

Figure 3

Fig 1

Shown in Figure 1, alignment of the T5 gene that codes for helicase with conserved segments of the helicase superfamily showed a sufficient alignment score and definitive evidence that this T5 protein is a helicase

"Table 2. Purification of the recombinant bacteriophage T5 dNMP kinase"..."these bacteriophages encode a deoxyribonucleoside monophosphate kinase (EC 2.7.4.12) catalyzing phosphorylation of 3 out of the 4 deoxyribonucleoside monophosphates included in its DNA–dTMP, dGMP,1 and hm-dCMP."

An enzyme assay determined one unit of deoxyribonucleotide kinase activity to be defined as catalyzing of the phosphorylation of 1 umol of 1 dNMP per minute

A mutation in A1 was found to prevent supression of pre-early gene transcription and is likely involved in the shut off of pre-early gene expression by some type of interaction with host RNA polymerase

Authors add and remove T4 replication proteins to prove the function of DNA primase. FIG. 6. The 61 protein alone can produce primers for initiating DNA synthesis.

Baseplate wedge protein gp25 cloned in vector and isolated by authors.

Gene subcloned and expressed in lambda vector.

Bacteriophage T4 dam gene expressed in plasmid by direct assay by authors. Fig 6

Authors show experimentally that by using a T4 dmd mutant that the dmd protein is serving as an anti-toxin for the T4 bacteriophage.