User:JamesMcDermott

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	BPT4:DMD	2016-04-21 19:35:25 CDT	GO:0048524 positive regulation of viral process (P)	PMID:22403819	ECO:0000315 mutant phenotype evidence used in manual assertion	Authors show experimentally that by using a T4 dmd mutant that the dmd protein is serving as an anti-toxin for the T4 bacteriophage.	challenge
updatedbyinstructor	BPT4:DMA	2016-04-24 19:50:44 CDT	GO:0009008 DNA-methyltransferase activity (F)	PMID:7782299	ECO:0000314 direct assay evidence used in manual assertion	Bacteriophage T4 dam gene expressed in plasmid by direct assay by authors. Fig 6	challenge
unacceptable	BPT4:GLRX	2016-04-24 21:16:49 CDT	GO:0015035 protein disulfide oxidoreductase activity (F)	PMID:3735493	ECO:0000314 direct assay evidence used in manual assertion	Gene subcloned and expressed in lambda vector.	challenge
unacceptable	BPT4:BP25	2016-04-24 21:48:13 CDT	GO:0098025 virus tail, baseplate (C)	PMID:3186452	ECO:0000314 direct assay evidence used in manual assertion	Baseplate wedge protein gp25 cloned in vector and isolated by authors.	challenge
updatedbyinstructor	BPT4:PRIM	2016-04-24 21:56:29 CDT	GO:0039693 viral DNA genome replication (P)	PMID:2422175	ECO:0000314 direct assay evidence used in manual assertion	Authors add and remove T4 replication proteins to prove the function of DNA primase. FIG. 6. The 61 protein alone can produce primers for initiating DNA synthesis.	challenge
unacceptable	BPT4:A0A023ZW86	2016-06-07 12:08:03 CDT	GO:0016021 integral component of membrane (C)	PMID:26081634	ECO:0000314 direct assay evidence used in manual assertion	Authors align full genome with NCBI T4 genome and this protein is identified. Authors deposit new genome in Genbank.	challenge
unacceptable	BPT4:FIB37	2016-06-07 12:42:19 CDT	GO:0098024 virus tail, fiber (C)	PMID:7338921	ECO:0000314 direct assay evidence used in manual assertion	Authors sequence tail fiber genes in assay of T4.	challenge
unacceptable	BPT4:VR2A	2016-06-08 10:40:35 CDT	GO:0020002 host cell plasma membrane (C)	PMID:2379817	ECO:0000315 mutant phenotype evidence used in manual assertion	Authors use missense mutations and sequence rIIA gene of bacteriophage T4.	challenge
updatedbyinstructor	BPT4:BP06	2016-06-08 11:03:55 CDT	GO:0098025 virus tail, baseplate (C)	PMID:2403438	ECO:0000314 direct assay evidence used in manual assertion	TABLE 3. Molecular weights and copy numbers of baseplate constituents	challenge

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unacceptable:6
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flagged:0

Annotations challenged by JamesMcDermott

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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