GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:2403438
Citation |
Watts, NR and Coombs, DH (1990) Structure of the bacteriophage T4 baseplate as determined by chemical cross-linking. J. Virol. 64:143-54 |
---|---|
Abstract |
We have carried out a series of reversible chemical cross-linking experiments using the reagent ethylene glycol-bis(succinimidylsuccinate) with the goal of determining the three-dimensional structure of the bacteriophage T4 baseplate. In a previous report, we investigated the near-neighbor contacts in baseplate precursors and substructures (N.R.M. Watts and D.H. Coombs, J. Virol. 63:2427-2436, 1989). Here we report completion of the analysis by examining finished baseplates and tails. Most of the previous contacts were confirmed, and we report several new contacts, including those within the central hub (gp5-gptd2, gp26-gptd), between the hub and the outer wedges (gp6-gp27(2], between baseplate and sheath (gp54-gp18), and between sheath and core (gp19-gp18). On the basis of this and other available information, a partial three-dimensional model of the baseplate is proposed. |
Links | |
Keywords |
Cross-Linking Reagents/pharmacology; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Escherichia coli/metabolism; Escherichia coli/ultrastructure; Immunoblotting; Models, Structural; Molecular Weight; Mutation; Succinimides/pharmacology; T-Phages/genetics; T-Phages/metabolism; T-Phages/ultrastructure; Viral Proteins/isolation & purification; Viral Proteins/metabolism; Viral Proteins/ultrastructure |
Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0098025: virus tail, baseplate |
ECO:0000314: |
C |
TABLE 3. Molecular weights and copy numbers of baseplate constituents |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.