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PMID:22403819

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Citation

Otsuka, Y and Yonesaki, T (2012) Dmd of bacteriophage T4 functions as an antitoxin against Escherichia coli LsoA and RnlA toxins. Mol. Microbiol. 83:669-81

Abstract

Enterohaemorrhagic Escherichia coli O157:H7 harbours a cryptic plasmid, pOSAK1, that carries only three ORFs: mobA (involved in plasmid mobilization), ORF1 and ORF2. Predicted proteins encoded by these two ORFs were found to share a weak homology with RnlA and RnlB, respectively, a toxin–antitoxin system encoded on the E. coli K-12 chromosome. Here, we report that lsoA (ORF1) encodes a toxin and lsoB (ORF2) an antitoxin. In spite of the homologies, RnlB and LsoB functioned as antitoxins against only their cognate toxins and not interchangeably with each other. Interestingly, T4 phage Dmd suppressed the toxicities of both RnlA and LsoA by direct interaction, the first example of a phage with an antitoxin against multiple toxins.

Links

PubMed

Keywords

Bacterial Toxins/antagonists & inhibitors; Bacterial Toxins/genetics; Bacteriophage T4/growth & development; Bacteriophage T4/metabolism; Bacteriophage T4/physiology; Escherichia coli K12/genetics; Escherichia coli O157/metabolism; Escherichia coli O157/virology; Escherichia coli Proteins/antagonists & inhibitors; Escherichia coli Proteins/genetics; Plasmids; Sequence Homology, Amino Acid; Viral Proteins/metabolism; Virus Replication

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:RNLA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P39232

F

Seeded From UniProt

complete

BPT4:DMD

GO:0048524: positive regulation of viral process

ECO:0000315:

P

Authors show experimentally that by using a T4 dmd mutant that the dmd protein is serving as an anti-toxin for the T4 bacteriophage.

complete
CACAO 11877

BPT4:DMD

GO:1903902: positive regulation of viral life cycle

ECO:0000315:

P

Figure 7A shows inhibited plaquing by T4 dmd knockouts compared to WT.

complete
CACAO 11994

Notes

See also

References

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