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PMID:3735493

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Citation

LeMaster, DM (1986) Nucleotide sequence and protein overproduction of bacteriophage T4 thioredoxin. J. Virol. 59:759-60

Abstract

The bacteriophage T4 thioredoxin gene was cloned and physically mapped to 47.6 kilobases from the reference BamHI site. The DNA sequence is consistent with that reported from earlier protein sequence studies. The gene was subcloned into a lambda pL overexpression vector which allowed for the isolation of approximately 5 mg/liter.

Links

PubMed PMC253257

Keywords

Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; Genes, Viral; T-Phages/genetics; T-Phages/metabolism; Thioredoxins/biosynthesis; Thioredoxins/genetics; Viral Proteins/biosynthesis; Viral Proteins/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPT4:GLRX

GO:0015035: protein disulfide oxidoreductase activity

ECO:0000314:

F

Gene subcloned and expressed in lambda vector.

complete
CACAO 11904

Notes

See also

References

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