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User:TaylorBourgeois

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableBPT5:A12016-04-26 12:27:07 CDTGO:0039657 suppression by virus of host gene expression (P)PMID:7033565ECO:0000315 mutant phenotype evidence used in manual assertion

A mutation in A1 was found to prevent supression of pre-early gene transcription and is likely involved in the shut off of pre-early gene expression by some type of interaction with host RNA polymerase

challenge
updatedbyinstructorBPT5:DNMK2016-04-26 19:02:48 CDTGO:0046939 nucleotide phosphorylation (P)PMID:14711503ECO:0000314 direct assay evidence used in manual assertion

"Table 2. Purification of the recombinant bacteriophage T5 dNMP kinase"..."these bacteriophages encode a deoxyribonucleoside monophosphate kinase (EC 2.7.4.12) catalyzing phosphorylation of 3 out of the 4 deoxyribonucleoside monophosphates included in its DNA–dTMP, dGMP,1 and hm-dCMP."

An enzyme assay determined one unit of deoxyribonucleotide kinase activity to be defined as catalyzing of the phosphorylation of 1 umol of 1 dNMP per minute

challenge
unacceptableBPT5:HEL102016-04-26 23:08:28 CDTGO:0004386 helicase activity (F)PMID:2547651ECO:0000247 sequence alignment evidence used in manual assertion

Shown in Figure 1, alignment of the T5 gene that codes for helicase with conserved segments of the helicase superfamily showed a sufficient alignment score and definitive evidence that this T5 protein is a helicase

challenge
updatedbyinstructorBPT5:CAPSD2016-04-26 23:35:25 CDTGO:0019028 viral capsid (C)PMID:24198424ECO:0000314 direct assay evidence used in manual assertion

Fig 1

challenge
updatedbyinstructorBPT5:EXO52016-05-02 23:18:55 CDTGO:0035312 5'-3' exodeoxyribonuclease activity (F)PMID:9874768ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3

challenge
acceptableBPT5:FIBL12016-05-05 17:05:12 CDTGO:0039638 lipopolysaccharide-mediated virion attachment to host cell (P)PMID:7045389ECO:0000315 mutant phenotype evidence used in manual assertion

As shown in Figure 1, the L-shaped tail fibers in T5 increase the absorption rate by reversibly binding to LPS on the host. The figure shows mutant hosts with more available O-antigens increase the absorption rate of T5 phage into the host.

challenge

acceptable:1
unacceptable:2
requires_changes:0
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Annotations challenged by TaylorBourgeois

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by TaylorBourgeois

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