BPT5:HEL10

Species (Taxon ID)	Escherichia phage T5 (Enterobacteria phage T5). (10726)
Gene Name(s)	D10
Protein Name(s)	Probable helicase D10 (ECO:0000312 with EMBL:AAX12052.1) Protein D10
External Links
UniProt	P11107
EMBL	AY543070 AY692264 AY587007
PIR	S01931
RefSeq	YP_006952.1
ProteinModelPortal	P11107
GeneID	2777605
KEGG	vg:2777605
Proteomes	UP000002107 UP000002141 UP000002503
GO	GO:0005524 GO:0003677 GO:0004386
Gene3D	3.40.50.300
InterPro	IPR006935 IPR014001 IPR001650 IPR027417
Pfam	PF00271 PF04851
SMART	SM00487 SM00490
SUPFAM	SSF52540
PROSITE	PS51192 PS51194

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0004386	helicase activity	PMID:2547651	ECO:0000247		PMID:2547651	F		Shown in Figure 1, alignment of the T5 gene that codes for helicase with conserved segments of the helicase superfamily showed a sufficient alignment score and definitive evidence that this T5 protein is a helicase	complete CACAO 11947
	GO:0008094	DNA-dependent ATPase activity	PMID:28009009	ECO:0000315			F		Figure 1b shows the mass of the eluted molecules to be consistent with those in DNA-dependent ATPase activity. Figure 1c shows enzymatic activity was only stimulated in the presence of duplex DNA. Figure 1d shows after cloning of D10 protein, D10-R389N (motif VI) was expressed and purified. When compared to wild type, a point mutation of the motif stopped all DNA-dependent ATPase activity.	complete CACAO 12443
	GO:1990163	ATP-dependent four-way junction helicase activity	PMID:28009009	ECO:0000314			F		The authors use a ATPase assay to determine if the helicase D10 can unwind complex branched DNA substrates. Figure 2b shows the ATP dependent unwinding of cruciform (four-way junction) DNA molecules by the T5 helicase gene D10.	complete CACAO 12594
	GO:0032508	DNA duplex unwinding	PMID:28009009	ECO:0000314			P		Figure 3 shows how d10 protein functions to unwind DNA. It uses partially homologous Holliday junction substrates and results in two products, including fork DNA and recombined duplex DNA. These gels prove that d10 can act as a DNA helicase.	complete CACAO 12644
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR006935	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR006935	F		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR006935	F		Seeded From UniProt	complete
enables	GO:0000166	nucleotide binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0547	F		Seeded From UniProt	complete
enables	GO:0004386	helicase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0347	F		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0067	F		Seeded From UniProt	complete
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References

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