User:Reillyer

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	HUMAN:ATG7	2014-04-03 11:40:25 CDT	GO:0006914 autophagy (P)	PMID:24681637	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2 indicates that cells treated to knockout atg7 showed no presence of LC3-II when treated with MitoQ. Wildtype cells treated with MitoQ showed sufficient levels of LC3-II. The presence of LC3-II is used to indicate autophagy. Thus, atg7 is necessary for MitoQ-induced autophagy.	challenge
updatedbyinstructor	?	2014-04-03 16:10:14 CDT	GO:0061304 retinal blood vessel morphogenesis (P)	PMID:23093773	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1a shows electron micrographs of murine retinas - a wildtype control, and a mutant 3 days after deleting the vegfa gene. The mutants lack choriocapillaris normally observed in controls. Thus, vegfa deletion results in complete ablation of the choriocapillaris.	challenge
updatedbyinstructor	CHICK:SOX21	2014-04-03 18:40:06 CDT	GO:0048839 inner ear development (P)	PMID:23071561	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 shows that sox21 promotes hair cell formation in vestibular patches during late embryonic stages. In vestibular patches, the majority of pTRE-eGFP transfected cells had support cell rather than hair cell morphologies. However, the majority of cells transfected with pTRE-eGFP-Sox21 had hair cell morphology.	challenge
unacceptable	CHICK:FPPS	2014-04-03 19:08:52 CDT	GO:0004337 geranyltranstransferase activity (F)	PMID:24206759	ECO:0000314 direct assay evidence used in manual assertion	According to figure 4, fdps genes were upregulated in abdominal fat, subcutaneous fat, and breast muscle tissues in WRR male chickens as opposed to XH male chickens. It can be inferred that the fast growing WRR chickens gain fat and muscle quicker as a result of abnormally upregulated genes, including fdps. Fdps plays a key role in fat and muscle development.	challenge
unacceptable	MOUSE:AZI1	2014-04-03 20:51:28 CDT	GO:0044441 ciliary part (C)	PMID:24550735	ECO:0000315 mutant phenotype evidence used in manual assertion	According to figure 4a, depletion of AZI1 reduced cilia formation by approximately 50%. Therefore, the azi1 gene positively influences cilia formation.	challenge
unacceptable	MOUSE:FGF15	2014-04-03 21:23:39 CDT	GO:0060557 positive regulation of vitamin D biosynthetic process (P)	PMID:24688219	ECO:0000314 direct assay evidence used in manual assertion	According to figure 1C, Fgf15 mRNA expression in the ileum was significantly increased in the mice fed the 0.1% Pi diet compared with groups fed the 0.6% or 1.2% Pi diet. Therefore, dietary Pi restriction up-regulates ileal Fgf15 gene expression.	challenge
updatedbyinstructor	YERPE:LCRF	2014-04-04 14:10:01 CDT	GO:0006355 regulation of transcription, DNA-templated (P)	PMID:24658611	ECO:0000315 mutant phenotype evidence used in manual assertion	According to figure 2a, activity of nine Ca2+ depletion induced promoters were decreased in cells with a mutant lcrf genotype as opposed to cells with a wildtype lcrf genotype. Thus, Ca2+ depletion induced genes are regulated by LcrF.	challenge
unacceptable	HUMAN:LYRIC	2014-04-04 14:38:26 CDT	GO:0016055 Wnt signaling pathway (P)	PMID:24348829	ECO:0000315 mutant phenotype evidence used in manual assertion	According to figure 2A, 2D, and 2E, silencing the aeg1 gene accounted for suppressed HepG2 proliferation and decreased colony formation when compared to the vector control. Thus, aeg-1 inhibits cell growth and promotes apoptosis in hepatoma HepG2 cells.	challenge

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unacceptable:4
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Annotations challenged by Reillyer

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Lapins19, MichSt14A 13	?	GO:0009631 - cold acclimation (Figure 4. CBF and COR genes are known members of a cold response pathway. The expression of these genes is induced by cold temperatures in wild type Arabidopsis. A cca1-11/lhy-21 double mutant exhibits basal levels of CBF and COR gene expression after exposure to cold temperatures.)	PMID:21471455	IMP: Inferred from Mutant Phenotype	challenge	C: 1
updatedbyinstructor	Adkinstr, MichSt14A 24	BACSU:PUTP	GO:0015193 - L-proline transmembrane transporter activity (F)	PMID:22139509	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

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