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Category:MichSt14A 13

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableCHLRE:B1B5J3Lapins19, MichSt14A 132014-04-05 13:51:30 CDTGO:0009649 entrainment of circadian clock (P)PMID:23898163ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5A. Wildtype and roc15 mutant Chlamydomonas was entrained to a 12:12 light:dark cycle. The cultures were transferred to constant darkness and their tufA expression was measured with luminescence. They were then expressed to 5 minute long pulses of light. The wild type Chlamydomonas responded to the light pulses, evident by a phase shift in tufA expression. The roc15 mutant Chlamydomonas did not exhibit this phase shift. This indicate that roc15 is essential for circadian regulated phase shifts and entrainment.

acceptableCHLRE:O48949Lapins19, MichSt14A 132014-04-05 13:36:28 CDTGO:0003756 protein disulfide isomerase activity (F)PMID:23475997ECO:0000314 direct assay evidence used in manual assertion

Figure 1D. An RNase activity assay shows that denatured RNase A was refolded in the presence of CPI2. The controls were fully active RNase A and RNase A without CPI2.

unacceptable?Lapins19, MichSt14A 132014-04-05 11:47:17 CDTGO:0009853 photorespiration (Figure 1C. In the shm1 mutant, photosynthetic rates are decreased in ambient CO2 conditions compared to wild-type photosynthetic rates. This indicates that shm1 is involved in photorespiration.)PMID:23551974IMP: Inferred from Mutant Phenotype
acceptablePHATC:B7FZ64Lapins19, MichSt14A 132014-04-03 11:21:33 CDTGO:0003913 DNA photolyase activity (F)PMID:19424294ECO:0000314 direct assay evidence used in manual assertion

Figure 2A. In vitro photorepair assay. A fragment of DNA had a (6-4) photoproduct at an MseI restriction. The photoproduct damage was repaired by PtCPF1. The restoration of the cut site allowed the fragment to be digested, resulting in a smaller fragment on a polyacrylamide gel. As a control, samples without the PtCPF1 protein could not be digested because the DNA damage was not repaired.

acceptableARATH:A1A6M1Lapins19, MichSt14A 132014-04-02 22:03:05 CDTGO:0003756 protein disulfide isomerase activity (F)PMID:23922206ECO:0000314 direct assay evidence used in manual assertion

Figure 10B. Denatured RNase A was refolded into its active form in the presence of pTAC5. The activity of the RNase A was tested at intervals throughout the assay. The percent of active RNase A increased after exposure to pTAC5. After 300 minutes, the RNase A activity was 30% higher than it was at the beginning of the assay.

unacceptable?Lapins19, MichSt14A 132014-04-02 16:29:24 CDTGO:0009631 cold acclimation (Figure 4. CBF and COR genes are known members of a cold response pathway. The expression of these genes is induced by cold temperatures in wild type Arabidopsis. A cca1-11/lhy-21 double mutant exhibits basal levels of CBF and COR gene expression after exposure to cold temperatures.)PMID:21471455IMP: Inferred from Mutant Phenotype

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