PMID:19424294

Citation	Coesel, S, Mangogna, M, Ishikawa, T, Heijde, M, Rogato, A, Finazzi, G, Todo, T, Bowler, C and Falciatore, A (2009) Diatom PtCPF1 is a new cryptochrome/photolyase family member with DNA repair and transcription regulation activity. EMBO Rep. 10:655-61
Abstract	Members of the cryptochrome/photolyase family (CPF) are widely distributed throughout all kingdoms, and encode photosensitive proteins that typically show either photoreceptor or DNA repair activity. Animal and plant cryptochromes have lost DNA repair activity and now perform specialized photoperceptory functions, for example, plant cryptochromes regulate growth and circadian rhythms, whereas mammalian and insect cryptochromes act as transcriptional repressors that control the circadian clock. However, the functional differentiation between photolyases and cryptochromes is now being questioned. Here, we show that the PtCPF1 protein from the marine diatom Phaeodactylum tricornutum shows 6-4 photoproduct repair activity and can act as a transcriptional repressor of the circadian clock in a heterologous mammalian cell system. Conversely, it seems to have a wide role in blue-light-regulated gene expression in diatoms. The protein might therefore represent a missing link in the evolution of CPFs, and act as a novel ultraviolet/blue light sensor in marine environments.
Links	PubMed PMC2711838 Online version:10.1038/embor.2009.59
Keywords	Algal Proteins/genetics; Algal Proteins/metabolism; Animals; COS Cells; Cercopithecus aethiops; Cryptochromes; DNA Repair; Diatoms/genetics; Flavoproteins/genetics; Flavoproteins/metabolism; Gene Expression Regulation; Phylogeny; Repressor Proteins/genetics; Repressor Proteins/metabolism; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PHATC:B7FZ64	enables	GO:0003913: DNA photolyase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
PHATC:B7FZ64		GO:0003913: DNA photolyase activity	ECO:0000314:		F		Figure 2A. In vitro photorepair assay. A fragment of DNA had a (6-4) photoproduct at an MseI restriction. The photoproduct damage was repaired by PtCPF1. The restoration of the cut site allowed the fragment to be digested, resulting in a smaller fragment on a polyacrylamide gel. As a control, samples without the PtCPF1 protein could not be digested because the DNA damage was not repaired.	complete CACAO 9764

References

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