GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

User:Leferej2

From GONUTS
Jump to: navigation, search

SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableBPT4:DDA2012-04-10 12:14:11 CDTGO:0004386 helicase activity (F)PMID:7806534ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows the ability of dda to perform helicase activity with or without the presence of proteins 41 or 59.

challenge
acceptableASF:Q9ICB82012-04-14 15:44:31 CDTGO:0045927 positive regulation of growth (P)PMID:10627538ECO:0000315 mutant phenotype evidence used in manual assertion

In Fig. 6, A shows smaller plaque sizes and this is the strain that contained the gene deletion.

challenge
requireschangesEBVB9:EAR2012-04-14 18:41:28 CDTGO:0042981 regulation of apoptotic process (P)PMID:12270148ECO:0000314 direct assay evidence used in manual assertion

Fig. 2 shows that in the presence of BHRF1 apoptosis is inhibited.

challenge
requireschangesECOLI:WZA2012-04-15 13:59:13 CDTGO:0015159 polysaccharide transmembrane transporter activity (F)PMID:14522970ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2-Wza derivatives were detected in the western immunoblots and the electron micrograph clearly shows capsule assembly on the surface of cells with Wza genes being expressed.

challenge
requireschangesPSEPA:Q7WYU82012-04-15 15:15:04 CDTGO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen (F)PMID:15262932ECO:0000314 direct assay evidence used in manual assertion

Fig 4. desZ molecular activity is shown by analysis of nuclear magnetic resonance at the various time intervals of growth that that enzymatic function of desZ is the incorporation of oxygen molecules on to the substrate

challenge
requireschangesPSEPA:PCYB2012-04-15 15:40:34 CDTGO:0006725 cellular aromatic compound metabolic process (P)PMID:15262932ECO:0000314 direct assay evidence used in manual assertion

Fig 5. lanes 8 and 11 show the ability of ligB mutants to metabolize 3-O-methylgallate as a carbon source.

challenge
requireschangesSTRHY:PAT2012-04-15 15:55:42 CDTGO:0009635 response to herbicide (P)PMID:16453789ECO:0000314 direct assay evidence used in manual assertion

Fig 3. The transgenic plants containing the bar gene show much higher rates of growth compared to the control plants when exposed to PPT herbicide.

challenge
requireschangesCVBQ:HEMA2012-04-15 17:00:03 CDTGO:0001681 sialate O-acetylesterase activity (F)PMID:11807232ECO:0000314 direct assay evidence used in manual assertion

Figure 2 demonstrates sialate O-acetylesterase activity

challenge
requireschanges9BACL:C4TP012012-04-15 17:07:08 CDTGO:0006725 cellular aromatic compound metabolic process (P)PMID:19717587ECO:0000314 direct assay evidence used in manual assertion

Fig 2. The conversion of PCA to 5CHMS is catalyzed by praA's enzymatic function. The optical density at different wavelength indicate that praA is in fact converting PCA (indicated by lower absorbance) into 5CHMS (indicated by higher absorbance).

challenge

acceptable:2
unacceptable:0
requires_changes:7
flagged:0

Annotations challenged by Leferej2

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesLulgjur2,
Team Gene-Yes
MOUSE:RFX2GO:0051091 - positive regulation of DNA-binding transcription factor activity (P)PMID:20003220ECO:0000021 physical interaction evidencechallengeC: 1

0 annotations fixed by Leferej2


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesBACSU:RIBC2012-03-29 12:17:35 CDTGO:0009231 riboflavin biosynthetic process (P)PMID:9473052ECO:0000315 mutant phenotype evidence used in manual assertion

FIG. 4 shows growth of mutant strain B. subtilis with over expression of ribC compared to wild type strains

challenge
acceptable9FLAV:Q8B4892012-04-01 15:14:54 CDTGO:0003968 RNA-directed RNA polymerase activity (F)PMID:21471248ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5 shows RNA polymerase activity through mutations in the Dengue Fever virus.

challenge
requireschanges9PARA:Q836002012-04-01 15:47:58 CDTGO:0004308 exo-alpha-sialidase activity (F)PMID:17223599ECO:0000314 direct assay evidence used in manual assertion

Fig. 3 shows the presence of sialic acid that was produced by using direct fluorescence.

challenge

acceptable:1
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Leferej2

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesLulgjur2,
Team Gene-Yes
MOUSE:RFX2GO:0051091 - positive regulation of DNA-binding transcription factor activity (P)PMID:20003220ECO:0000021 physical interaction evidencechallengeC: 1

0 annotations fixed by Leferej2