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Category:Team Buckminster Fullerenes

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SUPER BOWL ROUND Spring 2012

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableECOLI:ZUPTWrigh312, Team Buckminster Fullerenes2012-04-15 17:53:56 CDTGO:0006829 zinc ion transport (P)PMID:11790762ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3: Induced expression of zupT in mutants lacking all zinc transporters yielded much greater uptake of zinc than the controls lacking transduction by the zupT vector.

challenge
acceptableBACSU:YOKFWrigh312, Team Buckminster Fullerenes2012-04-15 17:41:25 CDTGO:0004518 nuclease activity (F)PMID:11584000ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5: DNase activity in cell-free extracts from wild-type and yokF deficient mutants exhibits nuclease activity.

challenge
requireschanges9BACL:C4TP01Leferej2, Team Buckminster Fullerenes2012-04-15 17:07:08 CDTGO:0006725 cellular aromatic compound metabolic process (P)PMID:19717587ECO:0000314 direct assay evidence used in manual assertion

Fig 2. The conversion of PCA to 5CHMS is catalyzed by praA's enzymatic function. The optical density at different wavelength indicate that praA is in fact converting PCA (indicated by lower absorbance) into 5CHMS (indicated by higher absorbance).

challenge
requireschangesCVBQ:HEMALeferej2, Team Buckminster Fullerenes2012-04-15 17:00:03 CDTGO:0001681 sialate O-acetylesterase activity (F)PMID:11807232ECO:0000314 direct assay evidence used in manual assertion

Figure 2 demonstrates sialate O-acetylesterase activity

challenge
requireschangesSTRHY:PATLeferej2, Team Buckminster Fullerenes2012-04-15 15:55:42 CDTGO:0009635 response to herbicide (P)PMID:16453789ECO:0000314 direct assay evidence used in manual assertion

Fig 3. The transgenic plants containing the bar gene show much higher rates of growth compared to the control plants when exposed to PPT herbicide.

challenge
requireschangesPSEPA:PCYBLeferej2, Team Buckminster Fullerenes2012-04-15 15:40:34 CDTGO:0006725 cellular aromatic compound metabolic process (P)PMID:15262932ECO:0000314 direct assay evidence used in manual assertion

Fig 5. lanes 8 and 11 show the ability of ligB mutants to metabolize 3-O-methylgallate as a carbon source.

challenge
acceptableORYSJ:HAK1Wrigh312, Team Buckminster Fullerenes2012-04-15 15:25:25 CDTGO:0006813 potassium ion transport (P)PMID:12376644ECO:0000314 direct assay evidence used in manual assertion

(Fig.7) K+ starved rice and yeast expressing OsHAK1 were grown on K+ containing media. The rate of depletion followed a Michaelis-Menten relationship in both rice and yeast mutants.

challenge
acceptableARATH:G2OX3Wrigh312, Team Buckminster Fullerenes2012-04-15 15:22:00 CDTGO:0009686 gibberellin biosynthetic process (P)PMID:7604047ECO:0000314 direct assay evidence used in manual assertion

Fig. 4: GA 20-oxidase activity, present in steps of the gibberellin biosynthesis pathway

challenge
requireschangesPSEPA:Q7WYU8Leferej2, Team Buckminster Fullerenes2012-04-15 15:15:04 CDTGO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen (F)PMID:15262932ECO:0000314 direct assay evidence used in manual assertion

Fig 4. desZ molecular activity is shown by analysis of nuclear magnetic resonance at the various time intervals of growth that that enzymatic function of desZ is the incorporation of oxygen molecules on to the substrate

challenge
requireschangesECOLI:WZALeferej2, Team Buckminster Fullerenes2012-04-15 13:59:13 CDTGO:0015159 polysaccharide transmembrane transporter activity (F)PMID:14522970ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2-Wza derivatives were detected in the western immunoblots and the electron micrograph clearly shows capsule assembly on the surface of cells with Wza genes being expressed.

challenge
updatedbyinstructorARATH:HMA3BWrigh312, Team Buckminster Fullerenes2012-04-15 13:26:51 CDTGO:0071585 detoxification of cadmium ion (P)PMID:19036834ECO:0000315 mutant phenotype evidence used in manual assertion

Fig.5: Overexpressed HMA3 in plant protoplasts tagged with BTC-5N/AM (a Cd/Zn fluorescing probe) conferred much greater fluorescence (particularly in vacuoles) when treated with Cd than wild-type plants.

challenge
requireschangesEBVB9:EARLeferej2, Team Buckminster Fullerenes2012-04-14 18:41:28 CDTGO:0042981 regulation of apoptotic process (P)PMID:12270148ECO:0000314 direct assay evidence used in manual assertion

Fig. 2 shows that in the presence of BHRF1 apoptosis is inhibited.

challenge
acceptableASF:Q9ICB8Leferej2, Team Buckminster Fullerenes2012-04-14 15:44:31 CDTGO:0045927 positive regulation of growth (P)PMID:10627538ECO:0000315 mutant phenotype evidence used in manual assertion

In Fig. 6, A shows smaller plaque sizes and this is the strain that contained the gene deletion.

challenge
acceptableBPT4:DDALeferej2, Team Buckminster Fullerenes2012-04-10 12:14:11 CDTGO:0004386 helicase activity (F)PMID:7806534ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows the ability of dda to perform helicase activity with or without the presence of proteins 41 or 59.

challenge


MSU Internal Competition

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschanges9PARA:Q83600Leferej2, Team Buckminster Fullerenes2012-04-01 15:47:58 CDTGO:0004308 exo-alpha-sialidase activity (F)PMID:17223599ECO:0000314 direct assay evidence used in manual assertion

Fig. 3 shows the presence of sialic acid that was produced by using direct fluorescence.

challenge
acceptable9FLAV:Q8B489Leferej2, Team Buckminster Fullerenes2012-04-01 15:14:54 CDTGO:0003968 RNA-directed RNA polymerase activity (F)PMID:21471248ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5 shows RNA polymerase activity through mutations in the Dengue Fever virus.

challenge
requireschangesARATH:AHM4BWrigh312, Team Buckminster Fullerenes2012-04-01 14:49:02 CDTGO:0005773 vacuole (C)PMID:15013746ECO:0000269 experimental evidence used in manual assertion

GFP fluorescence used to visualize HMA3 localization. Only found at vacuole (Fig. 4)

challenge
requireschangesBACSU:RIBCLeferej2, Team Buckminster Fullerenes2012-03-29 12:17:35 CDTGO:0009231 riboflavin biosynthetic process (P)PMID:9473052ECO:0000315 mutant phenotype evidence used in manual assertion

FIG. 4 shows growth of mutant strain B. subtilis with over expression of ribC compared to wild type strains

challenge

Pages in category "Team Buckminster Fullerenes"

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