PMID:10627538

Citation	Lewis, T, Zsak, L, Burrage, TG, Lu, Z, Kutish, GF, Neilan, JG and Rock, DL (2000) An African swine fever virus ERV1-ALR homologue, 9GL, affects virion maturation and viral growth in macrophages and viral virulence in swine.J. Virol. 74:1275-85
Abstract	The African swine fever virus (ASFV) genome contains a gene, 9GL, with similarity to yeast ERV1 and ALR genes. ERV1 has been shown to function in oxidative phosphorylation and in cell growth, while ALR has hepatotrophic activity. 9GL encodes a protein of 119 amino acids and was highly conserved at both nucleotide and amino acid levels among all ASFV field isolates examined. Monospecific rabbit polyclonal antibody produced to a glutathione S-transferase-9GL fusion protein specifically immunoprecipitated a 14-kDa protein from macrophage cell cultures infected with the ASFV isolate Malawi Lil-20/1 (MAL). Time course analysis and viral DNA synthesis inhibitor experiments indicated that p14 was a late viral protein. A 9GL gene deletion mutant of MAL (Delta9GL), exhibited a growth defect in macrophages of approximately 2 log(10) units and had a small-plaque phenotype compared to either a revertant (9GL-R) or the parental virus. 9GL affected normal virion maturation; virions containing acentric nucleoid structures comprised 90 to 99% of all virions observed in Delta9GL-infected macrophages. The Delta9GL virus was markedly attenuated in swine. In contrast to 9GL-R infection, where mortality was 100%, all Delta9GL-infected animals survived infection. With the exception of a transient fever response in some animals, Delta9GL-infected animals remained clinically normal and exhibited significant 100- to 10,000-fold reductions in viremia titers. All pigs previously infected with Delta9GL survived infection when subsequently challenged with a lethal dose of virulent parental MAL. Thus, ASFV 9GL gene deletion mutants may prove useful as live-attenuated ASF vaccines.
Links	PubMed
Keywords	African Swine Fever; African Swine Fever Virus; Amino Acid Sequence; Animals; Cells, Cultured; Conserved Sequence; DNA-Binding Proteins; Fungal Proteins; Gene Deletion; Immunization; Macrophages; Mitochondrial Proteins; Molecular Sequence Data; Mutation; Neoplasm Proteins; Oxidoreductases Acting on Sulfur Group Donors; Rats; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Swine; Viral Nonstructural Proteins; Viral Proteins; Virion; Virulence
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ASF:Q9ICB8		GO:0045927 : positive regulation of growth	ECO:0000315:		P		In Fig. 6, A shows smaller plaque sizes and this is the strain that contained the gene deletion.	complete CACAO 4695
ASF:Q9ICB8	involved_in	GO:0045927: positive regulation of growth	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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