User:Ksquires

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	HUMAN:HD	2014-01-30 15:29:36 CST	GO:0046825 regulation of protein export from nucleus (P)	PMID:12783847	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows the analysis of a putative nuclear localization signal of huntingtin in an in vivo assay of human HeLa epithelial cells. It does not contain a classic nuclear localization signal.	challenge
unacceptable	CHICK:AFAP1	2014-02-01 17:25:08 CST	GO:0003779 actin binding (F)	PMID:12134071	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows that rAFAP-110 bind to actin filaments directly by using gel electrophoresis, EM negative staining, and centrifugation.	challenge
unacceptable	CHICK:AFAP1	2014-02-01 17:31:07 CST	GO:0003779 actin binding (F)	PMID:12134071	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows that rAFAP-110 cross links actin through carboxyl terminal region by using a low-speed cosedimentation assay (A), and centrifugation of cross linked actin filaments versus free rAFAP-110 (B).	challenge
unacceptable	CHICK:AFAP1	2014-02-01 17:37:48 CST	GO:0003779 actin binding (F)	PMID:12134071	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 shows rAFAP cross links actin filaments by incubating them with rhodamine-phalloidin-labeled actin filaments.	challenge
unacceptable	CHICK:AFAP1	2014-02-01 18:15:19 CST	GO:0003779 actin binding (F)	PMID:12134071	ECO:0000314 direct assay evidence used in manual assertion	Figure 7 shows that PKC phosphorylation or leucine zipper deletion increases AFAp-110 ability to cross link actin filaments by incubating them with actin filaments (A) and incubating them with rhodamine-phalloidin-labeled actin filaments.	challenge

acceptable:0
unacceptable:5
requires_changes:0
flagged:0

Annotations challenged by Ksquires

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Kdiawara, Team Lobster Soul	HUMAN:HS90B	GO:0097435 - supramolecular fiber organization (P)	PMID:24466266	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 8
updatedbyinstructor	Trange, Team Kitten Me Right Meow	RAT:DNAS1	GO:0004536 - DNA nuclease activity (F)	PMID:8428592	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3
updatedbyinstructor	Malexander2, Team Pancakes At Nite	HUMAN:CIB1	GO:0005886 - plasma membrane (C)	PMID:20473878	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3 fixed by Grevever
updatedbyinstructor	Malexander2, Team Pancakes At Nite	HUMAN:CIB1	GO:0030291 - protein serine/threonine kinase inhibitor activity (F)	PMID:20473878	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
unacceptable	Cford, Team What the Fox Says	HUMAN:ENW1	GO:0001948 - glycoprotein binding (F)	PMID:15452578	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2

0 annotations fixed by Ksquires

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