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User:Ksquires

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableHUMAN:HD2014-01-30 15:29:36 CSTGO:0046825 regulation of protein export from nucleus (P)PMID:12783847ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows the analysis of a putative nuclear localization signal of huntingtin in an in vivo assay of human HeLa epithelial cells. It does not contain a classic nuclear localization signal.

challenge
unacceptableCHICK:AFAP12014-02-01 17:25:08 CSTGO:0003779 actin binding (F)PMID:12134071ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that rAFAP-110 bind to actin filaments directly by using gel electrophoresis, EM negative staining, and centrifugation.

challenge
unacceptableCHICK:AFAP12014-02-01 17:31:07 CSTGO:0003779 actin binding (F)PMID:12134071ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows that rAFAP-110 cross links actin through carboxyl terminal region by using a low-speed cosedimentation assay (A), and centrifugation of cross linked actin filaments versus free rAFAP-110 (B).

challenge
unacceptableCHICK:AFAP12014-02-01 17:37:48 CSTGO:0003779 actin binding (F)PMID:12134071ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows rAFAP cross links actin filaments by incubating them with rhodamine-phalloidin-labeled actin filaments.

challenge
unacceptableCHICK:AFAP12014-02-01 18:15:19 CSTGO:0003779 actin binding (F)PMID:12134071ECO:0000314 direct assay evidence used in manual assertion

Figure 7 shows that PKC phosphorylation or leucine zipper deletion increases AFAp-110 ability to cross link actin filaments by incubating them with actin filaments (A) and incubating them with rhodamine-phalloidin-labeled actin filaments.

challenge

acceptable:0
unacceptable:5
requires_changes:0
flagged:0

Annotations challenged by Ksquires

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorKdiawara,
Team Lobster Soul
HUMAN:HS90BGO:0097435 - supramolecular fiber organization (P)PMID:24466266ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 8
updatedbyinstructorTrange,
Team Kitten Me Right Meow
RAT:DNAS1GO:0004536 - deoxyribonuclease activity (F)PMID:8428592ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
updatedbyinstructorMalexander2,
Team Pancakes At Nite
HUMAN:CIB1GO:0005886 - plasma membrane (C)PMID:20473878ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3

fixed by Grevever
updatedbyinstructorMalexander2,
Team Pancakes At Nite
HUMAN:CIB1GO:0030291 - protein serine/threonine kinase inhibitor activity (F)PMID:20473878ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
unacceptableCford,
Team What the Fox Says
HUMAN:ENW1GO:0001948 - glycoprotein binding (F)PMID:15452578ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

0 annotations fixed by Ksquires

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