User:Biernate

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	STRMU:Q8DS93	2014-04-03 16:06:40 CDT	GO:0045892 negative regulation of transcription, DNA-templated (P)	PMID:17526837	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2a shows the expression levels of comC in the wild type samples and the comE mutant samples. The wild type samples only showed an increase in comC when grown in the presence of CSP, whereas comE mutants showed high expression levels of comC, even those that were not grown in the presence of CSP.	challenge
acceptable	PSEFL:G8Q5R9	2014-04-03 16:24:17 CDT	GO:0045892 negative regulation of transcription, DNA-templated (P)	PMID:24670089	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 shows the expression levels of 10 genes involved in iron homeostasis in both the wild type samples and amrZ mutants. In both of the iron sufficient and iron deficient mediums, the amrZ mutants displayed significantly higher levels of iron gene expression, suggesting that amrZ plays a role in suppressing these genes.	challenge
acceptable	FLAJ1:A5FIS8	2014-04-03 19:00:51 CDT	GO:0009306 protein secretion (P)	PMID:23667240	ECO:0000315 mutant phenotype evidence used in manual assertion	Figures 5 and 6 work together to suggest that GldK is needed to secrete the motility factors SprB and RemA. Figure 5 shows that RemA and SprB are still produced when the gene gldK is rendered nonfunctional, so gldK is not involved in their production. However, Figure 6 shows that gldK mutants are not motile, suggesting that RemA and SprB are not being secreted in the mutants, and thus GldK is involved in secreting the proteins.	challenge
acceptable	FLAJ1:A5FIS9	2014-04-03 19:06:33 CDT	GO:0009306 protein secretion (P)	PMID:23667240	ECO:0000315 mutant phenotype evidence used in manual assertion	Figures 5 and 6 work together to suggest that GldL is needed to secrete the motility factors SprB and RemA. Figure 5 shows that RemA and SprB are still produced when the gene gldL is rendered nonfunctional, so GldL is not involved in their production. However, Figure 6 shows that gldL mutants are not motile, suggesting that RemA and SprB are not being secreted in the mutants, and thus GldL is involved in secreting the proteins.	challenge
acceptable	FLAJ1:A5FIT0	2014-04-03 19:12:05 CDT	GO:0009306 protein secretion (P)	PMID:23667240	ECO:0000315 mutant phenotype evidence used in manual assertion	Figures 5 and 6 work together to suggest that GldM is needed to secrete the motility factors SprB and RemA. Figure 5 shows that RemA and SprB are still produced when the gene gldM is rendered nonfunctional, so GldM is not involved in their production. However, Figure 6 shows that gldM mutants are not motile, suggesting that RemA and SprB are not being secreted in the mutants, and thus GldM is involved in secreting the proteins.	challenge

acceptable:5
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Biernate

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	John.evenocheck, Team Just Name It Something	SINDV:POLS	GO:0039663 - membrane fusion involved in viral entry into host cell (P)	PMID:17483865	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
unacceptable	Gandii2, Team Protein Titans	HUMAN:A8KAQ8	GO:0010628 - positive regulation of gene expression (P)	PMID:22234890	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3
updatedbyinstructor	Drl5141, Team Protein Titans	MOUSE:SMO	GO:0040018 - positive regulation of multicellular organism growth (P)	PMID:24548465	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Biernate
updatedbyinstructor	Dalton.Hassler, Team Awesome 2014	ECOLI:CLPX	GO:0016887 - ATP hydrolysis activity (F)	PMID:11278349	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

1 annotations fixed by Biernate

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