GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
SINDV:POLS
Contents
| Species (Taxon ID) | Sindbis virus (SINV). (11034) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | Structural polyprotein
p130 Capsid protein Coat protein C p62 E3/E2 E3 protein Spike glycoprotein E3 E2 envelope glycoprotein Spike glycoprotein E2 6K protein E1 envelope glycoprotein Spike glycoprotein E1 | |
| External Links | ||
| UniProt | P03316 | |
| EMBL | V01403 J02363 M13818 AB372876 | |
| PIR | A03916 A25894 B03916 | |
| RefSeq | NP_062890.1 | |
| PDB | 1KXA 1KXB 1KXC 1KXD 1KXE 1KXF 1LD4 1SVP 1Z8Y 2SNV 2SNW 3J0F 3MUU 3MUW | |
| PDBsum | 1KXA 1KXB 1KXC 1KXD 1KXE 1KXF 1LD4 1SVP 1Z8Y 2SNV 2SNW 3J0F 3MUU 3MUW | |
| ProteinModelPortal | P03316 | |
| SMR | P03316 | |
| DIP | DIP-29032N | |
| MEROPS | S03.001 | |
| GeneID | 1502155 | |
| EvolutionaryTrace | P03316 | |
| PMAP-CutDB | P03316 | |
| Proteomes | UP000006710 | |
| GO | GO:0030430 GO:0020002 GO:0098029 GO:0016021 GO:0039619 GO:0019031 GO:0055036 GO:0004252 GO:0005198 GO:0075512 GO:0039654 GO:0061025 GO:0019062 | |
| Gene3D | 2.60.40.350 2.60.98.10 | |
| InterPro | IPR002548 IPR000936 IPR002533 IPR000336 IPR011998 IPR013754 IPR014756 IPR000930 IPR009003 | |
| Pfam | PF01589 PF00943 PF01563 PF00944 | |
| PRINTS | PR00798 | |
| SUPFAM | SSF50494 SSF56983 SSF81296 | |
| PROSITE | PS51690 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0061025 |
membrane fusion |
ECO:0000314 |
P |
Figure 3. |
complete | |||||
| GO:0098029 |
icosahedral viral capsid, spike |
ECO:0000314 |
C |
Figure 7. |
complete | |||||
|
Contributes to |
GO:0039619 |
T=4 icosahedral viral capsid |
ECO:0000315 |
C |
in figure 2.(lane 8,9,11,12)). "This suggests that in the absence of E3, the spike glycoproteins do not form heterodimeric complexes at any time after synthesis"(involved in virus assembly). Lobigs,M. et at.(1990). |
complete | ||||
| GO:0020002 |
host cell plasma membrane |
ECO:0000314 |
C |
In addition, subcellular localization of 6K-Myc was analyzed by immunofluorescence. The tagged polypeptide was detected at the plasma membrane and in a granular fashion in the perinuclear region, a region dense in ER membranes in Xenopus oocytes (Fig. 1). |
complete | |||||
| GO:0019062 |
virion attachment to host cell |
ECO:0000315 |
P |
Mutant strains of sindbis virus with insertion sequences in the PE2 glycoprotein gene have significantly reduced binding capabilities. PE2 cleavage is necessary for E2/E3 specific spike protein with E2 specific for receptor binding. See Fig. 3; Fig. 6; Fig. 7; Fig. 8 |
complete | |||||
| GO:0039663 |
membrane fusion involved in viral entry into host cell |
ECO:0000314 |
P |
Fig-1 shows how 6k makes the cell membrane more permeable by showing the location of tagged polypeptides in two different cells. One affected be the 6k protein and the other not. |
complete | |||||
| GO:0020002 |
host cell plasma membrane |
ECO:0000315 |
C |
Structural protein Sindbis virus, Figure 5 centrifugal separation of cellular membrane components from virus-infected cells, analyzed by western blot against virus proteins (specifically the TF protein) |
complete | |||||
|
enables |
GO:0044389 |
ubiquitin-like protein ligase binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0044389 |
ubiquitin-like protein ligase binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019062 |
virion attachment to host cell |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0020002 |
host cell plasma membrane |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0098029 |
icosahedral viral capsid, spike |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0061025 |
membrane fusion |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004252 |
serine-type endopeptidase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005198 |
structural molecule activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006508 |
proteolysis |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019028 |
viral capsid |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0055036 |
virion membrane |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0075509 |
endocytosis involved in viral entry into host cell |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0033644 |
host cell membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019028 |
viral capsid |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0030430 |
host cell cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019012 |
virion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0039663 |
membrane fusion involved in viral entry into host cell |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008236 |
serine-type peptidase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0039654 |
fusion of virus membrane with host endosome membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006508 |
proteolysis |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0020002 |
host cell plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0016032 |
viral process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0046718 |
viral entry into host cell |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0039619 |
T=4 icosahedral viral capsid |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019031 |
viral envelope |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0075512 |
clathrin-dependent endocytosis of virus by host cell |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019062 |
virion attachment to host cell |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008233 |
peptidase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0055036 |
virion membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 Smit, JM et al. (1999) Low-pH-dependent fusion of Sindbis virus with receptor-free cholesterol- and sphingolipid-containing liposomes. J. Virol. 73 8476-84 PubMed GONUTS page
- ↑ Lobigs, M et al. (1990) Function of Semliki Forest virus E3 peptide in virus assembly: replacement of E3 with an artificial signal peptide abolishes spike heterodimerization and surface expression of E1. J. Virol. 64 4346-55 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 Antoine, AF et al. (2007) The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes. J. Membr. Biol. 215 37-48 PubMed GONUTS page
- ↑ 4.0 4.1 Dubuisson, J & Rice, CM (1993) Sindbis virus attachment: isolation and characterization of mutants with impaired binding to vertebrate cells. J. Virol. 67 3363-74 PubMed GONUTS page
- ↑ Ramsey, J et al. (2017) Palmitoylation of Sindbis Virus TF Protein Regulates Its Plasma Membrane Localization and Subsequent Incorporation into Virions. J. Virol. 91 PubMed GONUTS page
- ↑ 6.0 6.1 Orvedahl, A et al. (2011) Image-based genome-wide siRNA screen identifies selective autophagy factors. Nature 480 113-7 PubMed GONUTS page
h
m
s
v
