GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:17483865

From GONUTS
Jump to: navigation, search
Citation

Antoine, AF, Montpellier, C, Cailliau, K, Browaeys-Poly, E, Vilain, JP and Dubuisson, J (2007) The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes. J. Membr. Biol. 215:37-48

Abstract

The Alphavirus Sindbis 6K protein is involved in several functions. It contributes to the processing and membrane insertion of E1 and PE2 viral envelope glycoproteins and to virus budding. It also permeabilizes Escherichia coli and mammalian cells. These viroporin-like properties have been proposed to help virus budding by modifying membrane permeabilities. We expressed Sindbis virus 6K cRNA in Xenopus oocytes to further characterize the effect of 6K on membrane conductances and permeabilization. Although no intrinsic channel properties were seen, cell shrinkage was observed within 24 h. Voltage-clamp experiments showed that 6K upregulated endogenous currents: a hyperpolarization-activated inward current (I (in)) and a calcium-dependent chloride current (I (Cl)). 6K was located at both the plasma and the endoplasmic reticulum membranes. The plasma membrane current upregulation likely results from disruption of the calcium homeostasis of the cell at the endoplasmic reticulum level. Indeed, 6K cRNA expression induced reticular calcium store depletion and capacitative calcium entry activation. By experimental modifications of the incubation medium, we showed that downstream of these events cell shrinkage resulted from a 6K -induced KCl efflux (I (Cl) upregulation leads to chloride efflux, which itself electrically drives potassium efflux), which was responsible for an osmotic water efflux. Our data confirm that 6K specifically triggers a sequential cascade of events that leads to cytoplasmic calcium elevation and cell permeabilization, which likely play a role in the Sindbis virus life cycle.

Links

PubMed Online version:10.1007/s00232-007-9003-6

Keywords

Alphavirus/physiology; Animals; Calcium/metabolism; Calcium Channels/metabolism; Chloride Channels/metabolism; Chlorides/metabolism; Female; Gene Expression Regulation, Viral/physiology; Oocytes/metabolism; Viral Envelope Proteins/biosynthesis; Viral Envelope Proteins/genetics; Viral Envelope Proteins/physiology; Xenopus; Xenopus laevis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SINDV:POLS

GO:0020002: host cell plasma membrane

ECO:0000314:

C

In addition, subcellular localization of 6K-Myc was analyzed by immunofluorescence. The tagged polypeptide was detected at the plasma membrane and in a granular fashion in the perinuclear region, a region dense in ER membranes in Xenopus oocytes (Fig. 1).

complete
CACAO 9610

SINDV:POLS

GO:0039663: membrane fusion involved in viral entry into host cell

ECO:0000314:

P

Fig-1 shows how 6k makes the cell membrane more permeable by showing the location of tagged polypeptides in two different cells. One affected be the 6k protein and the other not.

complete
CACAO 9926

SINDV:POLS

part_of

GO:0020002: host cell plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SFV:POLS

Contributes to

GO:0016021: integral component of membrane

ECO:0000314:

C

Fig-1 shows how 6k makes the cell membrane more permeable by showing the location of tagged polypeptides in two different cells. One affected be the 6k protein and the other not.

complete
CACAO 9561

See also

References

See Help:References for how to manage references in GONUTS.