PMID:23667240

Citation	Shrivastava, A, Johnston, JJ, van Baaren, JM and McBride, MJ (2013) Flavobacterium johnsoniae GldK, GldL, GldM, and SprA are required for secretion of the cell surface gliding motility adhesins SprB and RemA. J. Bacteriol. 195:3201-12
Abstract	Flavobacterium johnsoniae cells move rapidly over surfaces by gliding motility. Gliding results from the movement of adhesins such as SprB and RemA along the cell surface. These adhesins are delivered to the cell surface by a Bacteroidetes-specific secretion system referred to as the type IX secretion system (T9SS). GldN, SprE, SprF, and SprT are involved in secretion by this system. Here we demonstrate that GldK, GldL, GldM, and SprA are each also involved in secretion. Nonpolar deletions of gldK, gldL, or gldM resulted in the absence of gliding motility and in T9SS defects. The mutant cells produced SprB and RemA proteins but failed to secrete them to the cell surface. The mutants were resistant to phages that use SprB or RemA as a receptor, and they failed to attach to glass, presumably because of the absence of cell surface adhesins. Deletion of sprA resulted in similar but slightly less dramatic phenotypes. sprA mutant cells failed to secrete SprB and RemA, but cells remained susceptible to some phages and retained some limited ability to glide. The phenotype of the sprA mutant was similar to those previously described for sprE and sprT mutants. SprA, SprE, and SprT are needed for secretion of SprB and RemA but may not be needed for secretion of other proteins targeted to the T9SS. Genetic and molecular experiments demonstrate that gldK, gldL, gldM, and gldN form an operon and suggest that the proteins encoded by these genes may interact to form part of the F. johnsoniae T9SS.
Links	PubMed PMC3697645 Online version:10.1128/JB.00333-13
Keywords	Adhesins, Bacterial/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Secretion Systems; Flavobacterium/genetics; Flavobacterium/metabolism; Flavobacterium/physiology; Gene Knockout Techniques; Locomotion; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Operon
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
FLAJ1:A5FIS8	involved_in	GO:0009306: protein secretion	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
FLAJ1:A5FIS8		GO:0009306: protein secretion	ECO:0000315:		P		Figures 5 and 6 work together to suggest that GldK is needed to secrete the motility factors SprB and RemA. Figure 5 shows that RemA and SprB are still produced when the gene gldK is rendered nonfunctional, so gldK is not involved in their production. However, Figure 6 shows that gldK mutants are not motile, suggesting that RemA and SprB are not being secreted in the mutants, and thus GldK is involved in secreting the proteins.	complete CACAO 9802
FLAJ1:A5FIS9	involved_in	GO:0009306: protein secretion	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
FLAJ1:A5FIS9		GO:0009306: protein secretion	ECO:0000315:		P		Figures 5 and 6 work together to suggest that GldL is needed to secrete the motility factors SprB and RemA. Figure 5 shows that RemA and SprB are still produced when the gene gldL is rendered nonfunctional, so GldL is not involved in their production. However, Figure 6 shows that gldL mutants are not motile, suggesting that RemA and SprB are not being secreted in the mutants, and thus GldL is involved in secreting the proteins.	complete CACAO 9803
FLAJ1:A5FIT0	involved_in	GO:0009306: protein secretion	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
FLAJ1:A5FIT0		GO:0009306: protein secretion	ECO:0000315:		P		Figures 5 and 6 work together to suggest that GldM is needed to secrete the motility factors SprB and RemA. Figure 5 shows that RemA and SprB are still produced when the gene gldM is rendered nonfunctional, so GldM is not involved in their production. However, Figure 6 shows that gldM mutants are not motile, suggesting that RemA and SprB are not being secreted in the mutants, and thus GldM is involved in secreting the proteins.	complete CACAO 9805

References

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