Category:Team Designer Genes

Figure 4

Fig 3, using Figure 4 for binding.

Figure 1 A-C: Three different cell lines were transfected with plasmids for the over-expression of Na (which is the product of gene BRRF1) as well as control plasmids with no over-expression of Na. To see if Na initiated lysis in the cells in which Na was overproduced, immunoassays were performed to search for lytic cell products. The proteins searched for include BMRF1, Z protein, R protein, Na (through FLAG), and β-actin. In all three types of cells with plasmids over-expressing BRRF1, these lytic proteins were found in a greater amount than in the cells without the over-expression plasmids. Expression of BRRF1 leads to a product that affects maintenance of viral latency. The evidence code is IMP because there is over-expression of the gene. Presence of the gene product resulted

Interacting selectively and non-covalently with an E-box, a DNA motif with the consensus sequence CANNTG that is found in the promoters of a wide array of genes expressed in neurons, muscle and other tissues

Interacting selectively and non-covalently with a DNA region that regulates the transcription of a region of DNA, which may be a gene, cistron, or operon

Figure 6. Shows a favoring of aerobic glycolysis in the immortalized lymphoblastoid cell lines (LCL) when compared to both CD40+IL4 activated cells and newly Epstein-Barr Virus-infected B-cells. In addition to the increased levels of lactate and pyruvate, increased ROS levels were found in LCL's in comparison to the CD40+IL4 and EBV B-Cell cell lines.

BRLF1 is a transcriptional activator that mediates the switch from latent to lytic viral replication.

Figure 1 A-C: Three different cell lines were transfected with plasmids for the over-expression of Na (which is the product of gene BRRF1) as well as control plasmids with no over-expression of Na. To see if Na initiated lysis in the cells in which Na was overproduced, immunoassays were performed to search for lytic cell products. The proteins searched for include BMRF1, Z protein, R protein, Na (through FLAG), and β-actin. In all three types of cells with plasmids over-expressing BRRF1, these lytic proteins were found in a greater amount than in the cells without the over-expression plasmids. Expression of BRRF1 leads to a product that affects maintenance of viral latency. The evidence code is IMP because there is over-expression of the gene. Presence of the gene product resulted in an increase in specific lytic proteins measured by an immunoblot assay.

BRLF1 is a transcriptional activator that mediates the switch from latent to lytic viral replication.

Figure 1 A-C: Three different cell lines were transfected with plasmids for the over-expression of Na (which is the product of gene BRRF1) as well as control plasmids with no over-expression of Na. To see if Na initiated lysis in the cells in which Na was overproduced, immunoassays were performed to search for lytic cell products. The proteins searched for include BMRF1, Z protein, R protein, Na (through FLAG), and β-actin. In all three types of cells with plasmids over-expressing BRRF1, these lytic proteins were found in a greater amount than in the cells without the over-expression plasmids. Expression of BRRF1 leads to a product that affects maintenance of viral latency. The evidence code is IMP because there is over-expression of the gene. Presence of the gene product resulted in an increase in specific lytic proteins measured by an immunoblot assay.

Fig 1: DNA methylation enhances BZLF1 transactivation of most early lytic EBV promoters. This proves BZLF1 has a major affect on EBV entering the lytic cycle.

The Z2 element of the Z protein acts as a secondary sperm receptor and is expressed primary to ovulation of the oocyte. The Z2 element consists of 745 amino acids.

Fig. 1 An electron micrograph shows the internal structure of Zp.

As shown in Figure 2, LF2 represses Rta activation of four different EBV promoters