CACAO Spring 2014
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| unacceptable | EBVB9:RTA | 2014-03-23 14:16:42 CDT | GO:0019046 release from viral latency (P) | PMID:1 | ECO:0000315 mutant phenotype evidence used in manual assertion | BRLF1 is a transcriptional activator that mediates the switch from latent to lytic viral replication.
Figure 1 A-C: Three different cell lines were transfected with plasmids for the over-expression of Na (which is the product of gene BRRF1) as well as control plasmids with no over-expression of Na. To see if Na initiated lysis in the cells in which Na was overproduced, immunoassays were performed to search for lytic cell products. The proteins searched for include BMRF1, Z protein, R protein, Na (through FLAG), and β-actin. In all three types of cells with plasmids over-expressing BRRF1, these lytic proteins were found in a greater amount than in the cells without the over-expression plasmids. Expression of BRRF1 leads to a product that affects maintenance of viral latency. The evidence code is IMP because there is over-expression of the gene. Presence of the gene product resulted in an increase in specific lytic proteins measured by an immunoblot assay.
| challenge |
| unacceptable | EBVB9:RTA | 2014-03-23 14:28:16 CDT | GO:0019046 release from viral latency (P) | PMID:21325409 | ECO:0000315 mutant phenotype evidence used in manual assertion | BRLF1 is a transcriptional activator that mediates the switch from latent to lytic viral replication.
Figure 1 A-C: Three different cell lines were transfected with plasmids for the over-expression of Na (which is the product of gene BRRF1) as well as control plasmids with no over-expression of Na. To see if Na initiated lysis in the cells in which Na was overproduced, immunoassays were performed to search for lytic cell products. The proteins searched for include BMRF1, Z protein, R protein, Na (through FLAG), and β-actin. In all three types of cells with plasmids over-expressing BRRF1, these lytic proteins were found in a greater amount than in the cells without the over-expression plasmids. Expression of BRRF1 leads to a product that affects maintenance of viral latency. The evidence code is IMP because there is over-expression of the gene. Presence of the gene product resulted in an increase in specific lytic proteins measured by an immunoblot assay.
| challenge |
| unacceptable | EBVB9:RTA | 2014-03-30 20:14:06 CDT | GO:0019046 release from viral latency (P) | PMID:21325409 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 1 A-C: Three different cell lines were transfected with plasmids for the over-expression of Na (which is the product of gene BRRF1) as well as control plasmids with no over-expression of Na. To see if Na initiated lysis in the cells in which Na was overproduced, immunoassays were performed to search for lytic cell products. The proteins searched for include BMRF1, Z protein, R protein, Na (through FLAG), and β-actin. In all three types of cells with plasmids over-expressing BRRF1, these lytic proteins were found in a greater amount than in the cells without the over-expression plasmids. Expression of BRRF1 leads to a product that affects maintenance of viral latency. The evidence code is IMP because there is over-expression of the gene. Presence of the gene product resulted
| challenge |
acceptable:0
unacceptable:3
requires_changes:0
flagged:0
Annotations challenged by Mow11
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by Mow11
What do the icons mean in the status column?
