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Category:Team Anne O. Taters

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SUPER BOWL ROUND Spring 2012

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesARATH:NFXL2Claxtonk, Team Anne O. Taters2012-04-14 19:22:16 CDTGO:0009651 response to salt stress (P)PMID:22073231ECO:0000315 mutant phenotype evidence used in manual assertion

figure 3 shows that nfxl2 plants showed the most survival with wildtype plants not being able to survive a longer drought period but nfxl2-1 plants showed approximately a 60% survival rate.

challenge
requireschangesARATH:MOCOSClaxtonk, Team Anne O. Taters2012-04-14 18:25:26 CDTGO:0009688 abscisic acid biosynthetic process (P)PMID:22412184ECO:0000314 direct assay evidence used in manual assertion

figure 3 shows that Z35 (non transgenic plants) had higher levels of water loss over time compared to L5 and L8 plants (both transgenic plants) meaning that the transgenic plants has increased ABA production which lead to better drought tolerance and less water loss.

challenge
acceptableARATH:ICS1Giorda13, Team Anne O. Taters2012-04-10 08:11:57 CDTGO:0008909 isochorismate synthase activity (F)PMID:11734859ECO:0000247 sequence alignment evidence used in manual assertion

Fig. 2d indicates a clustal alignment of the Arabidpopsis ICS1 region with selected isochorismate synthases. Note the identical regions in black and the conserved regions in gray, as well as the critical regions shared for ICS activity (blue asterisk)

challenge
requireschangesERWAM:O54621Giorda13, Team Anne O. Taters2012-04-10 07:59:20 CDTGO:0050708 regulation of protein secretion (P)PMID:20569339ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 1 shows the protein content from DspF mutants that exhibit little to no DspA/E secretion (a commonly secreted Hrp- protein) in the culture supernatant.

challenge
acceptableXANEU:AVRB3Giorda13, Team Anne O. Taters2012-04-09 14:20:09 CDTGO:0009405 pathogenesis (P)PMID:12118879ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1a. Shows symptoms of a susceptible pepper leaf that was inoculated with either an empty vector Xanthomonas campestris pv. vesicatoria strain without AvrBs3 (no hypertrophy) or a strain that is expressing avrBs3 which clearly shows hypertrophy and pathogenesis. Fig 1b also shows the expression of avrBs3 in nicotiana clevlandii through agrobacterium mediated transformation which also elicits hypertrophy.

challenge
acceptablePSESM:HPAB2Giorda13, Team Anne O. Taters2012-04-09 14:18:38 CDTGO:0009405 pathogenesis (P)PMID:16792692ECO:0000315 mutant phenotype evidence used in manual assertion

Fig1. AvrPtoB inserted into vectors shows strong pathogenic response on bean and Arabidopsis compared to the empty vector control which only shows the hypersensitive response. Also the truncation of AvrPtoB from the c-terminus in different constructs leads to the eventual loss of pathogenesis on bean and Arabidopsis (1. b, c)

challenge
requireschangesARATH:TGA5Giorda13, Team Anne O. Taters2012-04-09 14:14:40 CDTGO:0009814 defense response, incompatible interaction (P)PMID:12383081ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 6 shows the antisense lines that overexpress TGA5 to be less susceptible to infection by p. parasitica than the wild type.

Also, Fig. 7 shows that this is independent of the Salicylate mediated defense pathway, as the defense response is observed in the nahG transgene background which abolishes SA/PR-1 mediated defense.

challenge
acceptableARATH:RIN4Giorda13, Team Anne O. Taters2012-04-09 14:13:10 CDTGO:0034051 negative regulation of plant-type hypersensitive response (P)PMID:11955429ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5a. shows the rin4 mutant to have less disease progression than the complement and the wild type. 5B. shows the number of spores from the pathogen to be much higher in the rin4 mutants. 5c. shows the expression of PR-1 a protein directly involved in the plant hypersensitive response is higher in rin4 mutants than it is in wild type (Ws-O) and rin4 complements.

challenge


MSU Internal Competition

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesARATH:RPM1Giorda13, Team Anne O. Taters2012-03-29 11:28:01 CDTGO:0009814 defense response, incompatible interaction (P)PMID:7638602ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2B. Complementation of rpm1 mutants with cosmid 1FB elicited hypersensitive response and inhibits growth of P. syringae DC3000, similar to the wild type controls. Empty vector mutants inoculated were unable to inhibit pathogen growth.

challenge
unacceptableHUMAN:PClaxtonk, Team Anne O. Taters2012-03-29 11:25:47 CDTGO:0006749 glutathione metabolic process (P)PMID:22065085ECO:0000316 genetic interaction evidence used in manual assertion

Figure 2 shows that the allele BEH2 is blue eye associated and is limited to Europe whereas alleles BEH1 and BEH3 are found on a global pattern.


challenge

Pages in category "Team Anne O. Taters"

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