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User:Giorda13

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableARATH:RIN42012-04-09 14:13:10 CDTGO:0034051 negative regulation of plant-type hypersensitive response (P)PMID:11955429ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5a. shows the rin4 mutant to have less disease progression than the complement and the wild type. 5B. shows the number of spores from the pathogen to be much higher in the rin4 mutants. 5c. shows the expression of PR-1 a protein directly involved in the plant hypersensitive response is higher in rin4 mutants than it is in wild type (Ws-O) and rin4 complements.

challenge
requireschangesARATH:TGA52012-04-09 14:14:40 CDTGO:0009814 defense response, incompatible interaction (P)PMID:12383081ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 6 shows the antisense lines that overexpress TGA5 to be less susceptible to infection by p. parasitica than the wild type.

Also, Fig. 7 shows that this is independent of the Salicylate mediated defense pathway, as the defense response is observed in the nahG transgene background which abolishes SA/PR-1 mediated defense.

challenge
acceptableARATH:ICS12012-04-10 08:11:57 CDTGO:0008909 isochorismate synthase activity (F)PMID:11734859ECO:0000247 sequence alignment evidence used in manual assertion

Fig. 2d indicates a clustal alignment of the Arabidpopsis ICS1 region with selected isochorismate synthases. Note the identical regions in black and the conserved regions in gray, as well as the critical regions shared for ICS activity (blue asterisk)

challenge
acceptablePSESM:HPAB22012-04-09 14:18:38 CDTGO:0009405 pathogenesis (P)PMID:16792692ECO:0000315 mutant phenotype evidence used in manual assertion

Fig1. AvrPtoB inserted into vectors shows strong pathogenic response on bean and Arabidopsis compared to the empty vector control which only shows the hypersensitive response. Also the truncation of AvrPtoB from the c-terminus in different constructs leads to the eventual loss of pathogenesis on bean and Arabidopsis (1. b, c)

challenge
acceptableXANEU:AVRB32012-04-09 14:20:09 CDTGO:0009405 pathogenesis (P)PMID:12118879ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1a. Shows symptoms of a susceptible pepper leaf that was inoculated with either an empty vector Xanthomonas campestris pv. vesicatoria strain without AvrBs3 (no hypertrophy) or a strain that is expressing avrBs3 which clearly shows hypertrophy and pathogenesis. Fig 1b also shows the expression of avrBs3 in nicotiana clevlandii through agrobacterium mediated transformation which also elicits hypertrophy.

challenge
requireschangesERWAM:O546212012-04-10 07:59:20 CDTGO:0050708 regulation of protein secretion (P)PMID:20569339ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 1 shows the protein content from DspF mutants that exhibit little to no DspA/E secretion (a commonly secreted Hrp- protein) in the culture supernatant.

challenge

acceptable:4
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Giorda13

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Giorda13


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesARATH:RPM12012-03-29 11:28:01 CDTGO:0009814 defense response, incompatible interaction (P)PMID:7638602ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2B. Complementation of rpm1 mutants with cosmid 1FB elicited hypersensitive response and inhibits growth of P. syringae DC3000, similar to the wild type controls. Empty vector mutants inoculated were unable to inhibit pathogen growth.

challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Giorda13

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Giorda13