PMID:20569339

Citation	Gaudriault, S, Paulin, JP and Barny, MA (2002) The DspB/F protein of Erwinia amylovora is a type III secretion chaperone ensuring efficient intrabacterial production of the Hrp-secreted DspA/E pathogenicity factor. Mol. Plant Pathol. 3:313-20
Abstract	Summary Erwinia amylovora is a Gram-negative bacterium responsible for fire blight, a necrotic disease affecting plants of the Rosaceae family. E. amylovora virulence is dependent on a functional type III secretion system. To date, four proteins have been shown to travel through this secretion system: HrpN, HrpW, HrpA, and DspA/E. Next to dspA/E, dspB/F encodes a small acidic protein sharing features similar to those of type III secretion chaperones described in animal systems. Here, we show that DspA/E was not secreted in a dspB/F background, while other known type III secreted proteins (HrpN, HrpW, and HrpA) remained secreted to wild-type level. A functional copy of dspB/F introduced in trans in a dspB/F background restored DspA/E secretion. Further analysis showed that DspA/E was not detected in a dspB/F background. This effect was post-transcriptional as the expression of a dspA/E::uidA transcriptional fusion was not reduced in a dspB/F background. Affinity blot analysis experiments demonstrated that DspB/F specifically interacts with DspA/E in vitro. Therefore, DspB/F acts as a specific DspA/E chaperone and DspB/F prevents intrabacterial DspA/E degradation. dspB/F mutants were found to retain some pathogenicity to pear seedlings. This phenotype contrasts with the non-pathogenic phenotype of the dspA/E mutant and suggests that the DspA/E protein still transits through the type III secretion machinery in a dspB/F mutant even though it is not detected in vitro.
Links	PubMed Online version:10.1046/j.1364-3703.2002.00124.x
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ERWAM:O54621		GO:0050708: regulation of protein secretion	ECO:0000315:		P		Fig. 1 shows the protein content from DspF mutants that exhibit little to no DspA/E secretion (a commonly secreted Hrp- protein) in the culture supernatant.	complete CACAO 4250

References

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