PMID:12118879

Citation	Marois, E, Van den Ackerveken, G and Bonas, U (2002) The xanthomonas type III effector protein AvrBs3 modulates plant gene expression and induces cell hypertrophy in the susceptible host. Mol. Plant Microbe Interact. 15:637-46
Abstract	Xanthomonas campestris pv. vesicatoria bacteria expressing the type III effector protein AvrBs3 induce a hypersensitive response in pepper plants carrying the resistance gene Bs3. Here, we report that infection of susceptible pepper and tomato plants leads to an AvrBs3-dependent hypertrophy of the mesophyll tissue. Agrobacterium-mediated transient expression of the avrBs3 gene in tobacco and potato plants resulted in a similar phenotype. Induction of hypertrophy was shown to depend on the repeat region, nuclear localization signals, and acidic transcription activation domain (AAD) of AvrBs3, suggesting that the effector modulates the host's transcriptome. To search for host genes regulated by AvrBs3 in an AAD-dependent manner, we performed a cDNA-amplified fragment length polymorphism analysis of pepper mRNA populations. Thirteen AvrBs3-induced transcripts were identified and confirmed by reverse transcriptase-polymerase chain reaction. Sequence analysis revealed homologies to auxin-induced and expansinlike genes, which play a role in cell enlargement. These results suggest that some of the AvrBs3-induced genes may be involved in hypertrophy development and that xanthomonads possess type III effectors that steer host gene expression.
Links	PubMed Online version:10.1094/MPMI.2002.15.7.637
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/physiology; Capsicum/cytology; Capsicum/genetics; DNA, Complementary; Gene Expression Regulation, Plant/drug effects; Gene Expression Regulation, Plant/physiology; Indoleacetic Acids/pharmacology; Lycopersicon esculentum/cytology; Lycopersicon esculentum/genetics; Molecular Sequence Data; Repetitive Sequences, Nucleic Acid; Xanthomonas campestris/genetics; Xanthomonas campestris/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
XANEU:AVRB3		GO:0009405: pathogenesis	ECO:0000315:		P		Fig 1a. Shows symptoms of a susceptible pepper leaf that was inoculated with either an empty vector Xanthomonas campestris pv. vesicatoria strain without AvrBs3 (no hypertrophy) or a strain that is expressing avrBs3 which clearly shows hypertrophy and pathogenesis. Fig 1b also shows the expression of avrBs3 in nicotiana clevlandii through agrobacterium mediated transformation which also elicits hypertrophy.	complete CACAO 4247
XANEU:AVRB3	involved_in	GO:0009405: pathogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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