User:Tgoh1

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	ECOLI:USPC	2012-09-23 16:35:33 CDT	GO:0009651 response to salt stress (P)	PMID:19101563	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4a: UspC influences kdpFABC expresison and production under salt stress.	challenge
updatedbyinstructor	ARATH:P2C56	2012-11-04 14:33:13 CST	GO:0006470 protein dephosphorylation (P)	PMID:22090030	ECO:0000314 direct assay evidence used in manual assertion	Addition of ABI1 led to dephosphorylation of pSnRK2.6 (Sup. Fig. 1A). When SnRK2.6 was incubated with the catalytic domain of ABI1, the smeared multiple bands (left side of SDS page figure) reduced to a single one corresponding to an apparent molecular mass of ~41kDA (right lane). This result suggests that SnRK2.6 kinase activity may be hindered via inhibition of ABI1. In addition, using spectrophotometric assayfor protein phosphatases, the kinetic parameters fo ABI1-catalyzed dephosphorylation of SnRK2.6 were deteremined (Fig. 1D), exhibiting a highly specific and efficient inhibition activity.	challenge
acceptable	ARATH:SRK2E	2012-11-04 14:54:48 CST	GO:0046777 protein autophosphorylation (P)	PMID:22090030	ECO:0000314 direct assay evidence used in manual assertion	MS analysis confirms that certain Ser/Thr residues were phosphorylated in recombinantly expressed SnRK2.6 (Supp. Fig. 1B). This analysis was performed after SnRK2.6 was purified after dephosphorylation by ABI1. The ratios of phosphorylated peptides to unmodified peptides (this data can elucidate the identity of potential phosphorylated residues of interest)were determined based on ion intensities of targeted peptides at different time points. One can see that S175 was the dominant phosphorylation site.	challenge
unacceptable	ARATH:DRE1B	2012-11-04 17:25:27 CST	GO:0009648 photoperiodism (P)	PMID:22927419	ECO:0000314 direct assay evidence used in manual assertion	CBF1 transcript levels at zeitgeber time 8 (ZT8) were about three to fivefold higher in short day (SD; 8 hr. light, 16 hr. dark) plants compared with long day (LD; 16 hr. light, 8 hr.dark) plants. These levels were measured via qRT-PCR (See Fig. S1)	challenge
unacceptable	ARATH:DRE1B	2012-11-18 15:52:23 CST	GO:0043565 sequence-specific DNA binding (F)	PMID:9023378	ECO:0000314 direct assay evidence used in manual assertion	Fig. 4. CBF1 binds to C-repeat/DRE.	challenge
unacceptable	ARATH:DRE1B	2012-11-18 15:52:23 CST	GO:0006950 response to stress (P)	PMID:9023378	ECO:0000314 direct assay evidence used in manual assertion	Fig. 6: Northern shows that low termperature/water deficit conditions do not change CBF1 transcript levels	challenge
updatedbyinstructor	MYCTU:WHIB1	2012-11-18 17:26:03 CST	GO:0045892 negative regulation of transcription, DNA-templated (P)	PMID:22464736	ECO:0000314 direct assay evidence used in manual assertion	Figure 2. Apo-WhiB1 inhibits transcription of groEL2 in vitro	challenge

acceptable:1
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by Tgoh1

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Vtran1, Team Smarter Who?	HAEDU:G1UBA5	GO:0009279 - cell outer membrane (C)	PMID:11401984	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3
requireschanges	Cbice1, Team Smarter Who?	ANTEL:Q9NH96	GO:0044403 - biological process involved in symbiotic interaction (P)	PMID:10908850	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
requireschanges	Cbice1, Team Smarter Who?	ARATH:Q8VYM7	GO:2000280 - regulation of root development (P)	PMID:22629280	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2
updatedbyinstructor	Cbice1, Team Smarter Who?	ARATH:BOB1	GO:0048461 - flower structural organization (P)	PMID:19571304	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
requireschanges	Cbice1, Team Smarter Who?	VIBF1:Q5DYR1	GO:2000146 - negative regulation of cell motility (P)	PMID:22233679	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2

0 annotations fixed by Tgoh1