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ARATH:P2C56

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Species (Taxon ID) Arabidopsis thaliana (Mouse-ear cress). (3702)
Gene Name(s) ABI1
Protein Name(s) Protein phosphatase 2C 56

AtPP2C56 Protein ABSCISIC ACID-INSENSITIVE 1 Protein phosphatase 2C ABI1 PP2C ABI1

External Links
UniProt P49597
EMBL U12856
X77116
X78886
AL049483
AL161564
CP002687
AY035073
AY142623
AK226529
PIR T04263
RefSeq NP_194338.1
UniGene At.21332
PDB 3JRQ
3KDJ
3NMN
PDBsum 3JRQ
3KDJ
3NMN
ProteinModelPortal P49597
SMR P49597
BioGrid 14001
DIP DIP-36706N
IntAct P49597
MINT MINT-8390807
PRIDE P49597
EnsemblPlants AT4G26080.1
GeneID 828714
KEGG ath:AT4G26080
TAIR AT4G26080
eggNOG COG0631
HOGENOM HOG000233896
InParanoid P49597
KO K14497
OMA ACEMARK
PhylomeDB P49597
BioCyc ARA:AT4G26080-MONOMER
EvolutionaryTrace P49597
Proteomes UP000006548
Genevestigator P49597
GO GO:0005737
GO:0005634
GO:0005886
GO:0046872
GO:0019901
GO:0004722
GO:0009738
GO:0009788
GO:0006470
GO:0009787
GO:0010119
GO:0009737
GO:0009409
GO:0009408
Gene3D 3.60.40.10
InterPro IPR001932
IPR000222
IPR015655
PANTHER PTHR13832
Pfam PF00481
SMART SM00331
SM00332
SUPFAM SSF81606
PROSITE PS01032

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0006470

protein dephosphorylation

PMID:22090030[1]

ECO:0000314

P

Addition of ABI1 led to dephosphorylation of pSnRK2.6 (Sup. Fig. 1A). When SnRK2.6 was incubated with the catalytic domain of ABI1, the smeared multiple bands (left side of SDS page figure) reduced to a single one corresponding to an apparent molecular mass of ~41kDA (right lane). This result suggests that SnRK2.6 kinase activity may be hindered via inhibition of ABI1. In addition, using spectrophotometric assayfor protein phosphatases, the kinetic parameters fo ABI1-catalyzed dephosphorylation of SnRK2.6 were deteremined (Fig. 1D), exhibiting a highly specific and efficient inhibition activity.

complete
CACAO 5621

GO:0006470

protein dephosphorylation

PMID:22090030[1]

ECO:0000314

P

Figure 1D shows results of a kinetics experiment that show that ABI1 dephosphorylates phospho-SnRK2.6 in vitro.

complete
CACAO 5951

GO:0004721

phosphoprotein phosphatase activity

PMID:22090030[1]

ECO:0000314

F

Figure 1D shows the results of a kinetics experiment that shows that ABI1 cleaves a phosphate off of the phosphoprotein pSnRK2.6

complete
CACAO 5952

enables

GO:0019900

kinase binding

PMID:24179127[2]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:Q38997

F

Seeded From UniProt

complete

enables

GO:0016791

phosphatase activity

PMID:24179127[2]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0009737

response to abscisic acid

PMID:25680457[3]

ECO:0000270

expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0009788

negative regulation of abscisic acid-activated signaling pathway

PMID:26443375[4]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0004721

phosphoprotein phosphatase activity

PMID:22090030[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0006470

protein dephosphorylation

PMID:22090030[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0019901

protein kinase binding

PMID:20385816[5]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:Q9ZSA2

F

Seeded From UniProt

complete

enables

GO:0019901

protein kinase binding

PMID:20385816[5]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:Q9M101

F

Seeded From UniProt

complete

involved_in

GO:0009788

negative regulation of abscisic acid-activated signaling pathway

PMID:18835996[6]

ECO:0000316

genetic interaction evidence used in manual assertion

UniProtKB:Q5BPS3

P

Seeded From UniProt

complete

involved_in

GO:0010119

regulation of stomatal movement

PMID:16614222[7]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0009788

negative regulation of abscisic acid-activated signaling pathway

PMID:16614222[7]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0009787

regulation of abscisic acid-activated signaling pathway

PMID:18298671[8]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0009737

response to abscisic acid

PMID:8492808[9]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0009409

response to cold

PMID:1834244[10]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0009409

response to cold

PMID:12228349[11]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0009408

response to heat

PMID:15923322[12]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

PMID:18298671[8]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0004722

protein serine/threonine phosphatase activity

PMID:9537523[13]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0009788

negative regulation of abscisic acid-activated signaling pathway

PMID:21873635[14]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN002302327
TAIR:locus:2005488
TAIR:locus:2164610

P

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:21873635[14]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN002493005
TAIR:locus:2080787
TAIR:locus:2168449
TAIR:locus:2203766

C

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

PMID:21873635[14]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN002493005
SGD:S000002164
TAIR:locus:2005488
TAIR:locus:2080787
TAIR:locus:2168449
UniProtKB:Q9T010

C

Seeded From UniProt

complete

enables

GO:0004724

magnesium-dependent protein serine/threonine phosphatase activity

PMID:21873635[14]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000352745
PomBase:SPAC4A8.03c
PomBase:SPCC4F11.02
RGD:620393
RGD:628812
SGD:S000000891

F

Seeded From UniProt

complete

enables

GO:0004722

protein serine/threonine phosphatase activity

PMID:21873635[14]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

FB:FBgn0086361
MGI:MGI:1919137
MGI:MGI:2139740
PANTHER:PTN000352745
PomBase:SPAC2G11.07c
PomBase:SPAC4A8.03c
PomBase:SPCC1223.11
PomBase:SPCC4F11.02
RGD:620128
RGD:620393
RGD:628812
RGD:631363
RGD:735028
SGD:S000000152
SGD:S000000329
SGD:S000000891
SGD:S000002133
SGD:S000002164
SGD:S000005616
TAIR:locus:2005488
TAIR:locus:2030230
TAIR:locus:2041444
TAIR:locus:2045678
TAIR:locus:2065046
TAIR:locus:2080787
TAIR:locus:2124784
TAIR:locus:2164610
TAIR:locus:2168449
UniProtKB:A0A1D8PRZ8
UniProtKB:O15297
UniProtKB:O15355
UniProtKB:O75688
UniProtKB:P35813
UniProtKB:P49593
UniProtKB:Q59MW1
UniProtKB:Q59PS6
UniProtKB:Q8IHY0
UniProtKB:Q8WY54
UniProtKB:Q9I757
UniProtKB:Q9P0J1
WB:WBGene00001412
dictyBase:DDB_G0276155

F

Seeded From UniProt

complete

enables

GO:0003824

catalytic activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR001932

F

Seeded From UniProt

complete

enables

GO:0004722

protein serine/threonine phosphatase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR015655

F

Seeded From UniProt

complete

involved_in

GO:0006470

protein dephosphorylation

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR015655

P

Seeded From UniProt

complete

enables

GO:0043169

cation binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR000222

F

Seeded From UniProt

complete

enables

GO:0004721

phosphoprotein phosphatase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:3.1.3.16

F

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1003
UniProtKB-SubCell:SL-0039

C

Seeded From UniProt

complete

enables

GO:0016787

hydrolase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0378

F

Seeded From UniProt

complete

involved_in

GO:0009738

abscisic acid-activated signaling pathway

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0938

P

Seeded From UniProt

complete

enables

GO:0004721

phosphoprotein phosphatase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0904

F

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963
UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete

part_of

GO:0016020

membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete

enables

GO:0046872

metal ion binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0479

F

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0539
UniProtKB-SubCell:SL-0191

C

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 1.4 Xie, T et al. (2012) Molecular mechanism for inhibition of a critical component in the Arabidopsis thaliana abscisic acid signal transduction pathways, SnRK2.6, by protein phosphatase ABI1. J. Biol. Chem. 287 794-802 PubMed GONUTS page
  2. 2.0 2.1 Rodrigues, A et al. (2013) ABI1 and PP2CA phosphatases are negative regulators of Snf1-related protein kinase1 signaling in Arabidopsis. Plant Cell 25 3871-84 PubMed GONUTS page
  3. Matsuoka, D et al. (2015) An abscisic acid inducible Arabidopsis MAPKKK, MAPKKK18 regulates leaf senescence via its kinase activity. Plant Mol. Biol. 87 565-75 PubMed GONUTS page
  4. Mitula, F et al. (2015) Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 56 2351-67 PubMed GONUTS page
  5. 5.0 5.1 Geiger, D et al. (2010) Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities. Proc. Natl. Acad. Sci. U.S.A. 107 8023-8 PubMed GONUTS page
  6. Zhang, Y et al. (2008) F-box protein DOR functions as a novel inhibitory factor for abscisic acid-induced stomatal closure under drought stress in Arabidopsis,. Plant Physiol. 148 2121-33 PubMed GONUTS page
  7. 7.0 7.1 Mishra, G et al. (2006) A bifurcating pathway directs abscisic acid effects on stomatal closure and opening in Arabidopsis. Science 312 264-6 PubMed GONUTS page
  8. 8.0 8.1 Moes, D et al. (2008) Nuclear localization of the mutant protein phosphatase abi1 is required for insensitivity towards ABA responses in Arabidopsis. Plant J. 54 806-19 PubMed GONUTS page
  9. Finkelstein, RR (1993) Abscisic acid-insensitive mutations provide evidence for stage-specific signal pathways regulating expression of an Arabidopsis late embryogenesis-abundant (lea) gene. Mol. Gen. Genet. 238 401-8 PubMed GONUTS page
  10. Gilmour, SJ & Thomashow, MF (1991) Cold acclimation and cold-regulated gene expression in ABA mutants of Arabidopsis thaliana. Plant Mol. Biol. 17 1233-40 PubMed GONUTS page
  11. Mantyla, E et al. (1995) Role of Abscisic Acid in Drought-Induced Freezing Tolerance, Cold Acclimation, and Accumulation of LT178 and RAB18 Proteins in Arabidopsis thaliana. Plant Physiol. 107 141-148 PubMed GONUTS page
  12. Larkindale, J et al. (2005) Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol. 138 882-97 PubMed GONUTS page
  13. Leube, MP et al. (1998) ABI1 of Arabidopsis is a protein serine/threonine phosphatase highly regulated by the proton and magnesium ion concentration. FEBS Lett. 424 100-4 PubMed GONUTS page
  14. 14.0 14.1 14.2 14.3 14.4 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page