GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:18835996

From GONUTS
Jump to: navigation, search
Citation

Zhang, Y, Xu, W, Li, Z, Deng, XW, Wu, W and Xue, Y (2008) F-box protein DOR functions as a novel inhibitory factor for abscisic acid-induced stomatal closure under drought stress in Arabidopsis,. Plant Physiol. 148:2121-33

Abstract

Guard cells, which form stoma in leaf epidermis, sense and integrate environmental signals to modulate stomatal aperture in response to diverse conditions. Under drought stress, plants synthesize abscisic acid (ABA), which in turn induces a rapid closing of stoma, to prevent water loss by transpiration. However, many aspects of the molecular mechanism for ABA-mediated stomatal closure are still not understood. Here, we report a novel negative regulator of guard cell ABA signaling, DOR, in Arabidopsis (Arabidopsis thaliana). The DOR gene encodes a putative F-box protein, a member of the S-locus F-box-like family related to AhSLF-S(2) and specifically interacting with ASK14 and CUL1. A null mutation in DOR resulted in a hypersensitive ABA response of stomatal closing and a substantial increase of drought tolerance; in contrast, the transgenic plants overexpressing DOR were more susceptible to the drought stress. DOR is strongly expressed in guard cells and suppressed by ABA treatment, suggesting a negative feedback loop of DOR in ABA responses. Double-mutant analyses of dor with ABA-insensitive mutant abi1-1 showed that abi1-1 is epistatic to dor, but no apparent change of phospholipase Dalpha1 was detected between the wild type and dor. Affymetrix GeneChip analysis showed that DOR likely regulates ABA biosynthesis under drought stress. Taken together, our results demonstrate that DOR acts independent of phospholipase Dalpha1 in an ABA signaling pathway to inhibit the ABA-induced stomatal closure under drought stress.

Links

PubMed PMC2593669 Online version:10.1104/pp.108.126912

Keywords

Abscisic Acid/metabolism; Abscisic Acid/pharmacology; Arabidopsis/drug effects; Arabidopsis/metabolism; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/physiology; Cullin Proteins; F-Box Proteins/genetics; F-Box Proteins/metabolism; F-Box Proteins/physiology; Mutation; Oligonucleotide Array Sequence Analysis; Plant Stomata/drug effects; Plant Stomata/metabolism; Plant Stomata/physiology; Signal Transduction/genetics; Stress, Physiological

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ASK14

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q5BPS3

F

Seeded From UniProt

complete

ARATH:DOR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q94AH6

F

Seeded From UniProt

complete

ARATH:DOR

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O81057

F

Seeded From UniProt

complete

ARATH:DOR

involved_in

GO:0009788: negative regulation of abscisic acid-activated signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P49597

P

Seeded From UniProt

complete

ARATH:DOR

acts_upstream_of_or_within

GO:0010119: regulation of stomatal movement

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DOR

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DOR

acts_upstream_of_or_within

GO:0009414: response to water deprivation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DOR

involved_in

GO:0010118: stomatal movement

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DOR

involved_in

GO:0009788: negative regulation of abscisic acid-activated signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DOR

involved_in

GO:0042631: cellular response to water deprivation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CUL1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q5BPS3

F

Seeded From UniProt

complete

ARATH:P2C56

involved_in

GO:0009788: negative regulation of abscisic acid-activated signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:Q5BPS3

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.