GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:10908850
| Citation |
Reynolds, WS, Schwarz, JA and Weis, VM (2000) Symbiosis-enhanced gene expression in cnidarian-algal associations: cloning and characterization of a cDNA, sym32, encoding a possible cell adhesion protein. Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 126:33-44 |
|---|---|
| Abstract |
Mutualistic endosymbioses between two partners are complex associations that are regulated by the genetic interactions of the partners. One important marine symbiosis is that between various cnidarians, such as corals and anemones, and their photosynthetic algal symbionts. We have been interested in characterizing cnidarian host genes that are expressed as a function of the symbiotic state, using the temperate sea anemone Anthopleura elegantissima as a model. In this study, we report on symbiosis-enhanced expression and synthesis of sym32 in anemones. We characterized the full-length sym32 cDNA, obtained by RT-PCR, and demonstrated, by semi-quantitative RT-PCR, that sym32 transcript was much more abundant in symbiotic than in non-symbiotic host anemone RNA. Further, using immunoblots, we determined that an antibody made to a sym32 fusion protein labeled a 32 kD band much more strongly in symbiotic compared to non-symbiotic anemone protein homogenates. Databank searches revealed that the sym32 deduced amino acid sequence shares significant homology with the fasciclin I (Fas I) family of homophilic cell adhesion proteins, present in a variety of organisms ranging from bacteria to humans. This strong homology with the Fas I family suggests that sym32 is involved in regulation of the symbiosis by mediating cell-cell interactions. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Antigens, CD95/genetics; Base Sequence; Cell Adhesion Molecules/biosynthesis; Cell Adhesion Molecules/chemistry; Cell Adhesion Molecules/genetics; Cloning, Molecular; DNA, Complementary/genetics; Eukaryota/physiology; Gene Expression; Molecular Sequence Data; RNA, Messenger/biosynthesis; Sea Anemones/genetics; Sea Anemones/metabolism; Sequence Homology, Amino Acid; Symbiosis/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0007155: cell adhesion |
ECO:0000247: |
UniProtKB:P82198
|
P |
Figure 2 shows a strong alignment of Sym32 to mouse beta Ig-h3, a known cell adhesion protein. |
complete | |||
| GO:0044403: symbiosis, encompassing mutualism through parasitism |
ECO:0000314: |
P |
Figure 4 shows upregulation of sym32 in symbiotic anemones over aposymbiotic anemones. |
complete | ||||
|
involved_in |
GO:0007155: cell adhesion |
ECO:0000247: sequence alignment evidence used in manual assertion |
UniProtKB:P82198 |
P |
Seeded From UniProt |
complete | ||
See also
References
See Help:References for how to manage references in GONUTS.
