User:Pawlos25

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	CRYNB:COX19	2014-04-01 13:47:30 CDT	GO:0031930 mitochondria-nucleus signaling pathway (P)	PMID:23345593	ECO:0000315 mutant phenotype evidence used in manual assertion	COX19 is a required for the transduction of a SCO-dependent redox signal from mitochondria to ATP7A. Figure 5A-C shows the knockdown of COX19 and PET191. Knockdown of COX19 reduces cellular copper levels when compared to parental lines, whereas PET191 knockdown does not affect copper levels. COX19 is critical for regulating cellular copper efflux.	challenge
unacceptable	HUMAN:F5H8D7	2014-04-01 15:35:48 CDT	GO:0006284 base-excision repair (P)	PMID:24556691	ECO:0000315 mutant phenotype evidence used in manual assertion	Scaffolding protein, XRCC1, is involved in the coordination of DNA repair by interacting with components of the BER and single-strand break repair pathways. Figure 1 shows that XRCC1 is downregulated in breast cancer patients. This demonstrates the possibility of XRCC1 to be targeted to enhance the effect of DNA damaging agents in BRCAX patients. This can allow an increased interaction with BER if XRCC1 is targeted to be upregulated which can increase effectiveness of BER.	challenge
updatedbyinstructor	SYNP2:RBS	2014-04-03 13:49:13 CDT	GO:0016984 ribulose-bisphosphate carboxylase activity (F)	PMID:6408075	ECO:0000314 direct assay evidence used in manual assertion	table 2 and figure 1b	challenge
acceptable	SCHPM:K7PD52	2014-04-03 14:41:40 CDT	GO:0005737 cytoplasm (C)	PMID:23145069	ECO:0000314 direct assay evidence used in manual assertion	Figure 2B - Assay of erh1+ transcription demonstrating gene product is found in the cytoplasm.	challenge
acceptable	SCHPM:K7PD52	2014-04-03 14:44:05 CDT	GO:0005654 nucleoplasm (C)	PMID:23145069	ECO:0000314 direct assay evidence used in manual assertion	Figure 2b - erh1+ transcription analysis providing evidence of gene product in nucleus with exception of nucleolus.	challenge
updatedbyinstructor	HUMAN:AT2B4	2014-04-06 10:20:17 CDT	GO:1902806 regulation of cell cycle G1/S phase transition (P)	PMID:24448801	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4. Primary carotid vascular smooth muscle cells (VSMC) from PMCA4 knockout mice showed G1-phase arrest when compared to wild-type plasma membrane calcium ATPase-4 (PMCA4) mice. When electroporated with PMCA4 constructs, knockout PMCA4 mice displayed wild-type phenotype and progressed through G1-phase, whereas mutant mice could not due to having only 10% of normal calcium efflux activity.	challenge
updatedbyinstructor	HUMAN:TLR9	2014-04-06 10:58:36 CDT	GO:1901895 negative regulation of calcium-transporting ATPase activity (P)	PMID:24610369	ECO:0000314 direct assay evidence used in manual assertion	Figure 3b demonstrates the measurement of Ca2+ ATPase activity of sarcoplasmic reticulum Ca2+ ATPase (SERCA2) with and without toll like receptor 9 (TLR9) stimulation. TLR9 stimulation showed to reduce SERCA2 uptake activity of calcium from cytosol to endoplasmic/sarcoplasmic reticulum (ER/SR) and regulate storage of calcium in ER/SR. Figure 4 demonstrates how the reduction of calcium by TLR9 inhibiting SERCA2 decreases mitochondrial ATP levels leading to increased cellular protection during an immune response.	challenge

acceptable:2
unacceptable:1
requires_changes:1
flagged:0

Annotations challenged by Pawlos25

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Grevever, MichSt14A 8	GEOSE:AXE2	GO:0046555 - acetylxylan esterase activity (F)	PMID:21994937	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Downeseh, MichSt14A 7	ANGCA:R4TUW8	GO:0004197 - cysteine-type endopeptidase activity (F)	PMID:24696276	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Rousepie, MichSt14A 46	HUMAN:MUC1	GO:0033629 - negative regulation of cell adhesion mediated by integrin (P)	PMID:7698991	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

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