PMID:7698991

Citation	Wesseling, J, van der Valk, SW, Vos, HL, Sonnenberg, A and Hilkens, J (1995) Episialin (MUC1) overexpression inhibits integrin-mediated cell adhesion to extracellular matrix components. J. Cell Biol. 129:255-65
Abstract	Episialin (MUC1) is a transmembrane molecule with a large mucin-like extracellular domain protruding high above the cell surface. The molecule is located at the apical side of most glandular epithelial cells, whereas in carcinoma cells it is often present at the entire surface and it is frequently expressed in abnormally large quantities. We have previously shown that overexpression of episialin reduces cell-cell interactions. Here we show that the integrin-mediated adhesion to extracellular matrix of transfectants of a melanoma cell line (A375), a transformed epithelial cell line (MDCK-ras-e) and a human breast epithelial cell line (HBL-100) is reduced by high levels of episialin. This reduction can be reversed by inducing high avidity of the beta 1 integrins by mAb TS2/16 (at least for beta 1-mediated adhesion). The adhesion can also be restored by redistribution of episialin on the cell surface by monoclonal antibodies into patches or caps. Similarly, capping of episialin on ZR-75-1 breast carcinoma cells, growing in suspension, caused adherence and spreading of these cells. We propose that there is a delicate balance between adhesion and anti-adhesion forces in episialin expressing cells, which can be shifted towards adhesion by strengthening the integrin-mediated adhesion, or towards anti-adhesion by increasing the level of expression of episialin.
Links	PubMed PMC2120361
Keywords	Animals; Base Sequence; Breast; Breast Neoplasms; Cell Adhesion; Cell Division; Cell Line; Cell Line, Transformed; DNA Primers; Dogs; Extracellular Matrix Proteins; Female; Genes, ras; Humans; Integrins/physiology; Kidney; Melanoma; Membrane Glycoproteins/biosynthesis; Molecular Sequence Data; Mucin-1; Mucins/biosynthesis; Neoplasm Proteins/biosynthesis; Polymerase Chain Reaction; Recombinant Proteins/biosynthesis; Sequence Deletion; Transfection; Tumor Cells, Cultured
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:MUC1		GO:0033629: negative regulation of cell adhesion mediated by integrin	ECO:0000314:		P		Figure 3 demonstrates percent adhesion to different extracellular matrix components. Figure 5 demonstrates the inhibition or induction of integrin-mediated adhesion by antibodies against integrin subunits or against episialin. These figures support the conclucsion that there needs to be a balance between adhesion and anti-adhesion forces in cells expressing episialin. This can be regulated by strengthening integrin-mediated adhesion or increasing level of episialin expression to inhibit cell adhesion.	complete CACAO 10072
HUMAN:MUC1	involved_in	GO:0033629: negative regulation of cell adhesion mediated by integrin	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.