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User:Hessa

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableMOUSE:AHI12011-10-23 17:53:32 CDTGO:0005929 cilium (C)PMID:20081859ECO:0000314 direct assay evidence used in manual assertion

Figure 1 (e): Endogenous Ahi1 localization is shown in red to the base of photoreceptor connecting cillium (acetylated-tubulin: green) in Ahi1 +/- section and is absent in Ahi1 -/- Cillium.

challenge
acceptableMOUSE:AHI12011-10-23 17:53:33 CDTGO:0046548 retinal rod cell development (P)PMID:20081859ECO:0000315 mutant phenotype evidence used in manual assertion

Supplementary figure 2 (a): Transmission electron microscopy showed complete absence of both rod and cone outer segments indicating Ahi1 role in rod and cone cell development. EM longitudinal views of representative Ahi1+/- control and multiple Ahi1-/- photoreceptor cilia demonstrate absence of outer segment disks. OS=outer segments, CC=connecting cilium (and indicated by black lines), brackets indicate OS in control and disorganized membranes in the Ahi1-/-, scale=1μm

challenge
acceptableMOUSE:AHI12011-10-23 17:55:17 CDTGO:0046549 retinal cone cell development (P)PMID:20081859ECO:0000315 mutant phenotype evidence used in manual assertion

Supplementary figure 2 (a): Transmission electron microscopy showed complete absence of both rod and cone outer segments by P10, indicating a role for Ahi1 in rod and cone cell development.

challenge
acceptableMOUSE:AHI12011-10-23 17:53:33 CDTGO:0042462 eye photoreceptor cell development (P)PMID:20081859ECO:0000314 direct assay evidence used in manual assertion

Figure 5 (d): The photoreceptors of Nphp1- /-mice formed outer segments but underwent gradual retinal degeneration, which was slightly evident at 2 months. Figure shows Nphp1+/+ and Nphp1-/- retina at 8 wks stained for opsin and nuclei (Hoescht 33342). Scale=20μm

challenge
acceptableMOUSE:AHI12011-11-06 15:35:47 CSTGO:0050708 regulation of protein secretion (P)PMID:18636121ECO:0000315 mutant phenotype evidence used in manual assertion

Using anti-Hap1 that recognizes endogenous Hap1 in PC12 cells of rat origin, we found that transfected Ahi1 increased the level of endogenous Hap1A and

Hap1B in a dose-dependent manner (Figure 3, F and G).

challenge
acceptableMOUSE:AHI12011-11-06 15:35:47 CSTGO:0010976 positive regulation of neuron projection development (P)PMID:18636121ECO:0000315 mutant phenotype evidence used in manual assertion

Expression of truncated Ahi1 with Hap1B inhibited neurite outgrowth of PC12 cells compared with cells

coexpressing full-length Ahi1. To confirm this inhibitory effect, we transfected full-length or truncated Ahi1 alone into PC12 cells (Figure 4D). Counting of the transfected cells revealed that 55.3% ± 3.5% of full-length Ahi1 and 15% ± 2.1% (mean ± SEM;

challenge
acceptableMOUSE:HAP12011-11-06 15:40:35 CSTGO:0022008 neurogenesis (P)PMID:18636121ECO:0000315 mutant phenotype evidence used in manual assertion

Supplemental Figure 4 (b): We observed a significant reduction in the number of BrdU-positive cells in the absence of Hap1.

challenge
acceptableMOUSE:HAP12011-11-06 15:40:35 CSTGO:0021549 cerebellum development (P)PMID:18636121ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5A: There was a reduction in the size of cerebellar folia in Hap1-null mice compared with littermate controls.

challenge
acceptableMOUSE:AHI12011-11-06 16:47:05 CSTGO:0045927 positive regulation of growth (P)PMID:18936234ECO:0000314 direct assay evidence used in manual assertion

Figure 1 B Table I.

challenge
requireschangesRAT:Q9ERV02011-11-20 17:29:34 CSTGO:0006979 response to oxidative stress (P)PMID:21696630ECO:0000314 direct assay evidence used in manual assertion

Figure 2: C & D

Figure 8: A

challenge
acceptableRAT:Q9ERV02011-11-20 17:24:34 CSTGO:0016301 kinase activity (F)PMID:21696630ECO:0000314 direct assay evidence used in manual assertion

Figure 2: C & D.

challenge
acceptableRAT:Q9ERV02011-11-20 17:29:35 CSTGO:0006916 anti-apoptosis (P)PMID:21696630ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5: B, C, D & E.

Figure 7: A, B, C & D.

challenge
acceptableRAT:KPCD2011-11-20 17:24:49 CSTGO:0034599 cellular response to oxidative stress (P)PMID:21696630ECO:0000314 direct assay evidence used in manual assertion

Figure 1 : A, B, C, D, & E.

challenge
acceptableRAT:KPCD2011-11-20 17:24:49 CSTGO:0006915 apoptosis (P)PMID:21696630ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1: F "PKCδ knockdown by RNAi almost comple- tely blocked the cell death induced by H2O2 at 120 min of treatment, demonstrating the pro-apoptotic function performed by PKCδ in N27 cells" .

challenge
acceptableRAT:KPCD2011-11-20 17:24:50 CSTGO:0042325 regulation of phosphorylation (P)PMID:21696630ECO:0000314 direct assay evidence used in manual assertion

Fig. 4D

challenge

acceptable:14
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Hessa

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
acceptableJmicG,
Team Mighty Morphin GO Annotators
HUMAN:REL2GO:0048552 - regulation of metalloenzyme activity (P)PMID:16049981ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
acceptableJmicG,
Team Mighty Morphin GO Annotators
HUMAN:REL2GO:0045766 - positive regulation of angiogenesis (P)PMID:16049981ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
unacceptableOherrera,
Team That Will Beat You!!!
EBOZM:NCAPGO:0016235 - aggresome (C)PMID:21223600ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3
acceptableLrodgers,
Team KremlinOnTheCrum
CRYNJ:PACCGO:0006351 - transcription, DNA-templated (P)PMID:20174553ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3

0 annotations fixed by Hessa