User:Hessa

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	MOUSE:AHI1	2011-10-23 17:53:32 CDT	GO:0005929 cilium (C)	PMID:20081859	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 (e): Endogenous Ahi1 localization is shown in red to the base of photoreceptor connecting cillium (acetylated-tubulin: green) in Ahi1 +/- section and is absent in Ahi1 -/- Cillium.	challenge
acceptable	MOUSE:AHI1	2011-10-23 17:53:33 CDT	GO:0046548 retinal rod cell development (P)	PMID:20081859	ECO:0000315 mutant phenotype evidence used in manual assertion	Supplementary figure 2 (a): Transmission electron microscopy showed complete absence of both rod and cone outer segments indicating Ahi1 role in rod and cone cell development. EM longitudinal views of representative Ahi1+/- control and multiple Ahi1-/- photoreceptor cilia demonstrate absence of outer segment disks. OS=outer segments, CC=connecting cilium (and indicated by black lines), brackets indicate OS in control and disorganized membranes in the Ahi1-/-, scale=1μm	challenge
acceptable	MOUSE:AHI1	2011-10-23 17:55:17 CDT	GO:0046549 retinal cone cell development (P)	PMID:20081859	ECO:0000315 mutant phenotype evidence used in manual assertion	Supplementary figure 2 (a): Transmission electron microscopy showed complete absence of both rod and cone outer segments by P10, indicating a role for Ahi1 in rod and cone cell development.	challenge
acceptable	MOUSE:AHI1	2011-10-23 17:53:33 CDT	GO:0042462 eye photoreceptor cell development (P)	PMID:20081859	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 (d): The photoreceptors of Nphp1- /-mice formed outer segments but underwent gradual retinal degeneration, which was slightly evident at 2 months. Figure shows Nphp1+/+ and Nphp1-/- retina at 8 wks stained for opsin and nuclei (Hoescht 33342). Scale=20μm	challenge
acceptable	MOUSE:AHI1	2011-11-06 15:35:47 CST	GO:0050708 regulation of protein secretion (P)	PMID:18636121	ECO:0000315 mutant phenotype evidence used in manual assertion	Using anti-Hap1 that recognizes endogenous Hap1 in PC12 cells of rat origin, we found that transfected Ahi1 increased the level of endogenous Hap1A and Hap1B in a dose-dependent manner (Figure 3, F and G).	challenge
acceptable	MOUSE:AHI1	2011-11-06 15:35:47 CST	GO:0010976 positive regulation of neuron projection development (P)	PMID:18636121	ECO:0000315 mutant phenotype evidence used in manual assertion	Expression of truncated Ahi1 with Hap1B inhibited neurite outgrowth of PC12 cells compared with cells coexpressing full-length Ahi1. To confirm this inhibitory effect, we transfected full-length or truncated Ahi1 alone into PC12 cells (Figure 4D). Counting of the transfected cells revealed that 55.3% ± 3.5% of full-length Ahi1 and 15% ± 2.1% (mean ± SEM;	challenge
acceptable	MOUSE:HAP1	2011-11-06 15:40:35 CST	GO:0022008 neurogenesis (P)	PMID:18636121	ECO:0000315 mutant phenotype evidence used in manual assertion	Supplemental Figure 4 (b): We observed a significant reduction in the number of BrdU-positive cells in the absence of Hap1.	challenge
acceptable	MOUSE:HAP1	2011-11-06 15:40:35 CST	GO:0021549 cerebellum development (P)	PMID:18636121	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5A: There was a reduction in the size of cerebellar folia in Hap1-null mice compared with littermate controls.	challenge
acceptable	MOUSE:AHI1	2011-11-06 16:47:05 CST	GO:0045927 positive regulation of growth (P)	PMID:18936234	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 B Table I.	challenge
requireschanges	RAT:Q9ERV0	2011-11-20 17:29:34 CST	GO:0006979 response to oxidative stress (P)	PMID:21696630	ECO:0000314 direct assay evidence used in manual assertion	Figure 2: C & D Figure 8: A	challenge
acceptable	RAT:Q9ERV0	2011-11-20 17:24:34 CST	GO:0016301 kinase activity (F)	PMID:21696630	ECO:0000314 direct assay evidence used in manual assertion	Figure 2: C & D.	challenge
acceptable	RAT:Q9ERV0	2011-11-20 17:29:35 CST	GO:0006916 anti-apoptosis (P)	PMID:21696630	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5: B, C, D & E. Figure 7: A, B, C & D.	challenge
acceptable	RAT:KPCD	2011-11-20 17:24:49 CST	GO:0034599 cellular response to oxidative stress (P)	PMID:21696630	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 : A, B, C, D, & E.	challenge
acceptable	RAT:KPCD	2011-11-20 17:24:49 CST	GO:0006915 apoptosis (P)	PMID:21696630	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1: F "PKCδ knockdown by RNAi almost comple- tely blocked the cell death induced by H2O2 at 120 min of treatment, demonstrating the pro-apoptotic function performed by PKCδ in N27 cells" .	challenge
acceptable	RAT:KPCD	2011-11-20 17:24:50 CST	GO:0042325 regulation of phosphorylation (P)	PMID:21696630	ECO:0000314 direct assay evidence used in manual assertion	Fig. 4D	challenge

acceptable:14
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Hessa

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	JmicG, Team Mighty Morphin GO Annotators	HUMAN:REL2	GO:0048552 - regulation of metalloenzyme activity (P)	PMID:16049981	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
acceptable	JmicG, Team Mighty Morphin GO Annotators	HUMAN:REL2	GO:0045766 - positive regulation of angiogenesis (P)	PMID:16049981	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2
unacceptable	Oherrera, Team That Will Beat You!!!	EBOZM:NCAP	GO:0016235 - aggresome (C)	PMID:21223600	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3
acceptable	Lrodgers, Team KremlinOnTheCrum	CRYNJ:PACC	GO:0006351 - DNA-templated transcription (P)	PMID:20174553	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3

0 annotations fixed by Hessa