User:Bruewera

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	MOUSE:CCD66	2012-04-10 21:02:51 CDT	GO:0050908 detection of light stimulus involved in visual perception (P)	PMID:21680557	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3: Ccdc66 -/- mouse at 3 months revealed a reduced a-wave amplitude under scotopic conditions and a reduced b-wave amplitude under photopic conditions on a background intensity of 25 cd/m^2.	challenge
acceptable	CANLF:F1PKQ6	2012-04-10 21:44:42 CDT	GO:0050908 detection of light stimulus involved in visual perception (P)	PMID:21521437	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 2 and Figure 3 show that the amplitudes of the 30Hz flicker response were significantly reduced in the dogs homozygous for the RPGRIP1 mutation compared to the control group.	challenge
requireschanges	CHICK:O57480	2012-04-12 11:50:31 CDT	GO:0004383 guanylate cyclase activity (F)	PMID:9448321	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows the analysis of GC1 protein level expression in normal and rd/rd (retinal dystrophy affected)chicken retina using western blot probed with anti-retGC pAb GC2.	challenge
acceptable	BOVIN:RPE65	2012-04-12 12:08:55 CDT	GO:0004744 retinal isomerase activity (F)	PMID:16096063	ECO:0000314 direct assay evidence used in manual assertion	Figure 4,5. Fig. 4 shows the absence of isomerase activity activity in pRPE65-transerase 293T-RC cell homogenates using atROL as substrate and the presence of activity in the same cells using atRP as substrate. pRPE65-transfected likes do show isomerase activity.	challenge
updatedbyinstructor	MOUSE:GUC2F	2012-04-12 12:26:12 CDT	GO:0050908 detection of light stimulus involved in visual perception (P)	PMID:17255100	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2B: At high intensity light stimulus, the a-wave and sensitivity of the scotopic GC2 (GUCY2F) -/- mouse were slightly reduced compared to the WT.	challenge
acceptable	HUMAN:EYS	2012-04-12 12:55:56 CDT	GO:0050908 detection of light stimulus involved in visual perception (P)	PMID:18976725	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 shows the constriction of the visual field in individuals affected with a homozygous p.Tyr3156X mutation in EYS and a non-recordable ERG or ERG responses in a rod-cone pattern. One patient (4C) also demonstrated a photoreceptor dystrophy.	challenge

acceptable:3
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Bruewera

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Belllesl, Team Lappish Hag's Love Potion	HUMAN:Q8N519	GO:0010467 - gene expression (P)	PMID:21980471	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
requireschanges	Napiera8, Team Lappish Hag's Love Potion	PSEOL:ALKK	GO:0031956 - medium-chain fatty acid-CoA ligase activity (F)	PMID:1453953	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
requireschanges	Co227488, Team Har Gobind Khorana	CANAL:Q5ALT5	GO:0031505 - fungal-type cell wall organization (Congo red (compound that interacts with chitin/interferes with cell wall construction) and caspofungin (inhibitor of the β-(1,3)-glucan synthase) were used on msb2 wildtype and mutant phenotypes to determine sensibility based on msb2 presence. ​Fig.1A,1A, MSB2 deletion resulted in Congo red sensitivity (250 μg/ml) as opposed to wildtype)	PMID:19542310	EXP: Inferred from Experiment	challenge	C: 1

0 annotations fixed by Bruewera

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	CANFA:PDE6A	2012-03-29 11:34:07 CDT	GO:0050908 detection of light stimulus involved in visual perception (P)	PMID:18775863	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4: Average dark adapted a- and b- ERG wave amplitudes from mutant PDE6A puppies peaked at three weeks of age and were significantly smaller than control puppies of the same age. Mean light adapted a-wave amplitudes of affected dogs were lower than normal dogs at 3 weeks of age and decreased with increasing age.	challenge
requireschanges	CANFA:E7AIM5	2012-04-01 08:11:05 CDT	GO:0050908 detection of light stimulus involved in visual perception (P)	PMID:20806078	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3: Establishes ADAM9 mutation in dogs affected with crd3 in this study. Figure 4: ADAM9 mutation is crd3 affected dogs causes a frame shift mutation resulting in a premature stop codon at base 6 of exon 17. Figure 1:By 15 months of age ERG dysfunction is detected as reduced 30Hz flicker cone responses in dogs affected with crd3 due to ADAM9 mutation, and at later ages by continued deterioration of cone and rod responses in these affected dogs.	challenge

acceptable:0
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Bruewera

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Belllesl, Team Lappish Hag's Love Potion	HUMAN:Q8N519	GO:0010467 - gene expression (P)	PMID:21980471	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
requireschanges	Napiera8, Team Lappish Hag's Love Potion	PSEOL:ALKK	GO:0031956 - medium-chain fatty acid-CoA ligase activity (F)	PMID:1453953	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Bruewera