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PMID:17255100
| Citation |
Baehr, W, Karan, S, Maeda, T, Luo, DG, Li, S, Bronson, JD, Watt, CB, Yau, KW, Frederick, JM and Palczewski, K (2007) The function of guanylate cyclase 1 and guanylate cyclase 2 in rod and cone photoreceptors. J. Biol. Chem. 282:8837-47 |
|---|---|
| Abstract |
Retinal guanylate cyclases 1 and 2 (GC1 and GC2) are responsible for synthesis of cyclic GMP in rods and cones, but their individual contributions to phototransduction are unknown. We report here that the deletion of both GC1 and GC2 rendered rod and cone photoreceptors nonfunctional and unstable. In the rod outer segments of GC double knock-out mice, guanylate cyclase-activating proteins 1 and 2, and cyclic GMP phosphodiesterase were undetectable, although rhodopsin and transducin alpha-subunit were mostly unaffected. Outer segment membranes of GC1-/- and GC double knock-out cones were destabilized and devoid of cone transducin (alpha- and gamma-subunits), cone phosphodiesterase, and G protein-coupled receptor kinase 1, whereas cone pigments were present at reduced levels. Real time reverse transcription-PCR analyses demonstrated normal RNA transcript levels for the down-regulated proteins, indicating that down-regulation is posttranslational. We interpret these results to demonstrate an intrinsic requirement of GCs for stability and/or transport of a set of membrane-associated phototransduction proteins. |
| Links |
PubMed PMC2043484 Online version:10.1074/jbc.M610369200 |
| Keywords |
Amino Acid Sequence; Animals; Electroretinography/methods; Gene Deletion; Guanylate Cyclase/genetics; Guanylate Cyclase/physiology; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, Mutant Strains; Molecular Sequence Data; Receptors, Cell Surface/genetics; Receptors, Cell Surface/physiology; Retinal Cone Photoreceptor Cells/metabolism; Retinal Rod Photoreceptor Cells/metabolism; Rod Cell Outer Segment |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0050908: detection of light stimulus involved in visual perception |
ECO:0000315: |
P |
Figure 2B: At high intensity light stimulus, the a-wave and sensitivity of the scotopic GC2 (GUCY2F) -/- mouse were slightly reduced compared to the WT. |
complete | ||||
|
involved_in |
GO:0050908: detection of light stimulus involved in visual perception |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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