User:Amstutzc

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	CHLRE:STT7	2014-04-03 11:07:49 CDT	GO:0004674 protein serine/threonine kinase activity (F)	PMID:12624266	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 shows that stt7 Chlamydomonas reinhardtii mutants have increased maximal fluorescence compared to wild type under conditions that would induce state II transition. This mutant phenotype is rescued when complimented with the Stt7 gene. Thylakoid protein fractions with radiolabeled phosphate under state I and state II transition show that the phosphorylation status increases in transition II in wild type and rescued cells, but not in the stt7 mutants. Western blotting of similar fractions reacted with phosphor-threonine antiserum showed similar results. Since the antiserum will only bind to phosphate groups on threonine residues, the protein must be a threonine/serine kinase.	challenge
requireschanges	?	2014-04-03 12:45:24 CDT	GO:0046422 violaxanthin de-epoxidase activity (Figure 6 shows that under low light the pool of xanthophyll pigments in wild type Chlamydomonas reinhardtii is primarily composed of violxanthin. Under high light conditions, violaxanthin is epoxidated to antheraxanthin and zeaxanthin. However in the mutant NPQ1, the xanthin pool under high light remains primarily violaxanthin. Therefore the mutant is lacking violaxanthin epoxidase activity.)	PMID:12237386	IMP: Inferred from Mutant Phenotype		challenge
requireschanges	ARATH:ZEP	2014-04-03 13:04:01 CDT	GO:0010028 xanthophyll cycle (P)	PMID:12237386	ECO:0000315 mutant phenotype evidence used in manual assertion	The assignment of this protein to an activity is an infererence from the mutant phenotype related to accumulation of different compounds "the Npq- phenotype of npq2 co- segregated with the accumulation of zeaxanthin and absence of antheraxanthin, violaxanthin, and neoxanthin (data not shown)." According to figure 6, the xanthophyll pool in wild type cells in low light is primarily violaxanthin. In high light this violaxanthin decreases, producing antheraxanthin and zeaxanthin. However, the amount of zeaxanthin in the NPQ2 mutant remains the same when under low light. There is no production of antheraxanin or violaxanthin. This is due to the lack of zeaxanthin epoxidase activity.	challenge
requireschanges	?	2014-04-03 20:53:19 CDT	GO:0005634 nucleus (In supplemental figure 4, a GUS reporter was tagged to PIF1. The same cells were also stained with a DNA stain, DAPI, which localizes the nucleus. When the two images were superimposed, the GUS-PIF1 was localized only to the region that was also stained with DAPI, indicating PIF1 is in the nucleus.)	PMID:15448264	IDA: Inferred from Direct Assay		challenge
acceptable	CHLRE:A1YSB4	2014-04-04 10:14:46 CDT	GO:0009579 thylakoid (C)	PMID:19574473	ECO:0000314 direct assay evidence used in manual assertion	In Figure 5, wild type, rep27, and rep27 complimented cells were fractionated into soluble and membrane portions and analyzed for the presence of REP27. The western blot analysis detected REP27 in the total membrane and total extract fractions for the wild type and complemented cells. However no detection was found in the soluble fraction. Figure 6 isolated thylakoid memebranes and again looked for the presence of REP27 in the membrane and soluble fractions. REP27 was present in all membrane fractions but was absent from the soluble. These two figures indicate that the REP27 protein is localized to the thylakoid membranes in the chloroplast.	challenge

acceptable:2
unacceptable:0
requires_changes:3
flagged:0

Annotations challenged by Amstutzc

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Readettd, MichSt14A 19	YEAST:BAP2	GO:0015192 - L-phenylalanine transmembrane transporter activity (F)	PMID:24701347	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
unacceptable	Downeseh, MichSt14A 7	AEDAE:Q206L4	GO:0035049 - obsolete juvenile hormone acid methyltransferase activity (F)	PMID:24697753	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
unacceptable	Astephens2, Team Bacon Pancakes	HUMAN:FADD	GO:0031264 - death-inducing signaling complex (C)	PMID:7536190	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3

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