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PMID:12624266
| Citation |
Depège, N, Bellafiore, S and Rochaix, JD (2003) Role of chloroplast protein kinase Stt7 in LHCII phosphorylation and state transition in Chlamydomonas. Science 299:1572-5 |
|---|---|
| Abstract |
Photosynthetic organisms adapt to changes in light quality by redistributing light excitation energy between two photosystems through state transition. This reorganization of antenna systems leads to an enhanced photosynthetic yield. Using a genetic approach in Chlamydomonas reinhardtii to dissect the signal transduction pathway of state transition, we identified a chloroplast thylakoid-associated serine-threonine protein kinase, Stt7, that has homologs in land plants. Stt7 is required for the phosphorylation of the major light-harvesting protein (LHCII) and for state transition. |
| Links |
PubMed Online version:10.1126/science.1081397 |
| Keywords |
Algal Proteins/chemistry; Algal Proteins/genetics; Algal Proteins/metabolism; Alleles; Amino Acid Sequence; Animals; Arabidopsis/enzymology; Arabidopsis/genetics; Catalytic Domain; Chlamydomonas reinhardtii/enzymology; Chlamydomonas reinhardtii/genetics; Chlamydomonas reinhardtii/metabolism; Chloroplasts/enzymology; Cosmids; DNA, Complementary; Expressed Sequence Tags; Fluorescence; Genes; Genetic Complementation Test; Light; Molecular Sequence Data; Mutation; Oxidation-Reduction; Phosphorylation; Photosynthesis; Photosynthetic Reaction Center Complex Proteins/metabolism; Protein-Serine-Threonine Kinases/chemistry; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Thylakoids/enzymology; Transcription, Genetic; Transformation, Genetic |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0004674: protein serine/threonine kinase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0004674: protein serine/threonine kinase activity |
ECO:0000315: |
F |
Figure 1 shows that stt7 Chlamydomonas reinhardtii mutants have increased maximal fluorescence compared to wild type under conditions that would induce state II transition. This mutant phenotype is rescued when complimented with the Stt7 gene. Thylakoid protein fractions with radiolabeled phosphate under state I and state II transition show that the phosphorylation status increases in transition II in wild type and rescued cells, but not in the stt7 mutants. Western blotting of similar fractions reacted with phosphor-threonine antiserum showed similar results. Since the antiserum will only bind to phosphate groups on threonine residues, the protein must be a threonine/serine kinase. |
complete | ||||
See also
References
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