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GO:0090435protein localization to nuclear envelopePMID:15448264IDA: Inferred from Direct Assay P
This annotation made on page:
By: Amstutzc (group MichSt14A 1) on 2014-04-03 20:53:19 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeTernesch,
MichSt14A 22
2014-04-07 16:40:54 CDT

Since GUS-PIF1 was localized only to the region that was also stained with DAPI, and DAPI localizes the nucleus, the Go term should be GO:0034504 protein localization to nucleus instead of GO:0090435 protein localization to nuclear envelope.

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Suzialeksander2014-05-22 14:11:33 CDTYou need to be an instructor to view these notes.Requires Changes
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Suzialeksander2014-04-18 16:25:48 CDTYou need to be an instructor to view these notes.Requires Changes
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DanielRenfro2014-04-07 16:40:54 CDT

This annotation has been flagged because it has been edited since last assessment ||GO:0090435||protein localization to nuclear envelope||PMID:15448264||IDA: Inferred from Direct Assay||||P||In supplemental figure 4, a GUS reporter was tagged to PIF1. The same cells were also stained with a DNA stain, DAPI, which localizes the nucleus. When the two images were superimposed, the GUS-PIF1 was localized only to the region that was also stained with DAPI, indicating PIF1 is in the nucleus.||complete

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Ratiujer2014-04-06 18:21:32 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
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