User:Ternesch

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	MOUSE:ALDH2	2014-04-03 19:21:35 CDT	GO:0008106 alcohol dehydrogenase (NADP+) activity (F)	PMID:24651616	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 shows that protein ALDH2 has decreased myocardial activity in mice given a high-fat diet/ low-dose streptozotocin. Figure 8 shows activity of ALDH2 correlates inversely with cardiomyocyte hypertrophy.	challenge
unacceptable	MOUSE:CEBPA	2014-04-03 22:36:16 CDT	GO:0006631 fatty acid metabolic process (P)	PMID:24691027	ECO:0000314 direct assay evidence used in manual assertion	Figure 8. Oxidative metabolism is impaired in the skeletal muscle of chow-fed MαKO mice. Skeletal muscle (quadriceps) was collected from 6-7 months old, chow-fed mice after overnight fasting. The expression of genes related to FA oxidation, muscle types, and energy expenditure were measured by Q-PCR (n=5 per group) (a). Proteins related to FA oxidation in skeletal muscle were measured by Western blotting.	challenge
updatedbyinstructor	MOUSE:JAK2	2014-04-03 23:41:47 CDT	GO:0035166 post-embryonic hemopoiesis (P)	PMID:23544085	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3: B) Representative peripheral blood films from both genotypes showing the presence of anemia, thrombocytopenia, and poikilocytosis in the Jak2 cKO mice, but not the controls. (C) Histologic sections of spleen found an abnormal splenic architecture in the Jak2 cKO mice characterized by atrophied and disorganized white pulp.(D) Representative bone marrow sections from both genotypes indicating increased adipose deposits in the Jak2 cKO mice, but not the controls. (E) Representative liver sections showing diffuse centrolobular vacuolar degeneration in the Jak2 cKO tissue, but not in the control tissue.	challenge
unacceptable	MOUSE:B3GN5	2014-04-05 13:08:24 CDT	GO:0001574 ganglioside biosynthetic process (P)	PMID:2998480	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 a. Results from three different tissues in adult mice. In the wild-type (+/+) genotype, Lc3 synthase gene expression was detected mainly in spleen and was weakly positive in brain and liver. Lc3 synthase expression was decreased in the heterozygote (+/-) and completely knocked out in the homozygote (-/-). Lc3 synthase is a step in the ganglioside biosynthetic process.	challenge
updatedbyinstructor	MOUSE:PON3	2014-04-05 23:35:28 CDT	GO:0032929 negative regulation of superoxide anion generation (P)	PMID:22441669	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 C shows that PON3 diminishes mitochondrial superoxide formation when compared to PON3 knockout cells.	challenge

acceptable:0
unacceptable:3
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flagged:0

Annotations challenged by Ternesch

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Amstutzc, MichSt14A 1	?	GO:0005634 - nucleus (In supplemental figure 4, a GUS reporter was tagged to PIF1. The same cells were also stained with a DNA stain, DAPI, which localizes the nucleus. When the two images were superimposed, the GUS-PIF1 was localized only to the region that was also stained with DAPI, indicating PIF1 is in the nucleus.)	PMID:15448264	IDA: Inferred from Direct Assay	challenge	C: 1
updatedbyinstructor	Ternesch, MichSt14A 22	MOUSE:JAK2	GO:0035166 - post-embryonic hemopoiesis (P)	PMID:23544085	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2 fixed by Ternesch

1 annotations fixed by Ternesch

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