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PMID:24701347
| Citation |
Sáenz, DA, Chianelli, MS and Stella, CA (2014) L-Phenylalanine Transport in Saccharomyces cerevisiae: Participation of GAP1, BAP2, and AGP1. J Amino Acids 2014:283962 |
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| Abstract |
We focused on the participation of GAP1, BAP2, and AGP1 in L-phenylalanine transport in yeast. In order to study the physiological functions of GAP1, BAP2, and AGP1 in L-phenylalanine transport, we examined the kinetics, substrate specificity, and regulation of these systems, employing isogenic haploid strains with the respective genes disrupted individually and in combination. During the characterization of phenylalanine transport, we noted important regulatory phenomena associated with these systems. Our results show that Agp1p is the major transporter of the phenylalanine in a gap1 strain growing in synthetic media with leucine present as an inducer. In a wild type strain grown in the presence of leucine, when ammonium ion was the nitrogen source, Bap2p is the principal phenylalanine carrier. |
| Links |
PubMed Online version:10.1155/2014/283962 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0015192: L-phenylalanine transmembrane transporter activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0015192: L-phenylalanine transmembrane transporter activity |
ECO:0000315: |
F |
Figure 1 shows phenylalanine transport in wild type cells grown on medium with proline as the sole nitrogen source is catalyzed by Gap1. Gap1 activity is absent when ammonium ion is used as a nitrogen source. |
complete | ||||
| GO:0015192: L-phenylalanine transmembrane transporter activity |
ECO:0000315: |
F |
Figure 1 shows BAP2 to be the major permease responsible for high affinity phenylalanine transport |
complete | ||||
|
enables |
GO:0015192: L-phenylalanine transmembrane transporter activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0015192: L-phenylalanine transmembrane transporter activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0015192: L-phenylalanine transmembrane transporter activity |
ECO:0000315: |
F |
Figure 1 shows AGP1 has low affinity phenylalanine permease activity |
complete | ||||
See also
References
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