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User:Readettd

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableYEAST:GAP12014-04-06 11:50:50 CDTGO:0015192 L-phenylalanine transmembrane transporter activity (F)PMID:24701347ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows phenylalanine transport in wild type cells grown on medium with proline as the sole nitrogen source is catalyzed by Gap1. Gap1 activity is absent when ammonium ion is used as a nitrogen source.

challenge
acceptableYEAST:BAP22014-04-06 12:02:26 CDTGO:0015192 L-phenylalanine transmembrane transporter activity (F)PMID:24701347ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows BAP2 to be the major permease responsible for high affinity phenylalanine transport

challenge
acceptableYEAST:AGP12014-04-06 12:12:42 CDTGO:0015192 L-phenylalanine transmembrane transporter activity (F)PMID:24701347ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows AGP1 has low affinity phenylalanine permease activity

challenge
acceptable9ROSI:C0LU172014-04-06 13:36:34 CDTGO:0004503 monophenol monooxygenase activity (F)PMID:24613318ECO:0000314 direct assay evidence used in manual assertion

Table 3 shows a monophenolase activity of kcat 20.8 s^-1 towards L-tyrosine

challenge
updatedbyinstructor9ROSI:C0LU172014-04-06 13:39:44 CDTGO:0036263 L-DOPA monooxygenase activity (F)PMID:24613318ECO:0000314 direct assay evidence used in manual assertion

Table 3 shows a diphenolase activity of kcat 199.3 s^-1 towards L-dopa

challenge
unacceptableFRATT:Q5NGM82014-04-06 15:15:40 CDTGO:0016021 integral component of membrane (C)PMID:24593131ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the CapA-frGFP fusion protein, which was used to identify CapA as an integral inner-membrane protein with both the N- and C- termini oriented towards the cytoplasm.

challenge
unacceptableRHOS4:Q3J2P52014-04-06 15:50:59 CDTGO:0015250 water channel activity (F)PMID:24497982ECO:0000314 direct assay evidence used in manual assertion

Figure 7 shows the average water permeability of the aquaporin to be 1.23±0.43×10−12 cm3/s

challenge

acceptable:4
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Readettd

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorDrl5141,
Team Protein Titans
MOUSE:STAT1GO:0060337 - type I interferon-mediated signaling pathway (P)PMID:24598055ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
updatedbyinstructorDrl5141,
Team Protein Titans
MOUSE:STAT2GO:0060337 - type I interferon-mediated signaling pathway (P)PMID:24598055ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
requireschangesMnxj39,
Team blindmice
HUMAN:NEU1GO:0090303 - positive regulation of wound healing (P)PMID:24205344ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3
requireschangesMegans,
Team Rubby ducky
VIBCB:LUXOGO:0000156 - phosphorelay response regulator activity (F)PMID:10027982ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Readettd

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