User:Megans

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	PHOPO:LUXC	2014-03-01 19:03:21 CST	GO:0050062 long-chain-fatty-acyl-CoA reductase activity (F)	PMID:6833298	ECO:0000314 direct assay evidence used in manual assertion	Figure 2C - Thin layer chromatography. Shows that when the protein of interest is incubated with radiolabeled tetradecanoyl-CoA (an intermediate in fatty acid metabolism) and NADPH, an aldehyde product is produced and low amounts of free fatty acids are detected. This indicates that the Acyl-CoA reductase reduces the tetradecanoyl-CoA intermediate to the aldehyde substrate.	challenge
acceptable	PHOPO:LUXD	2014-03-22 14:48:04 CDT	GO:0016746 transferase activity, transferring acyl groups (F)	PMID:6147345	ECO:0000314 direct assay evidence used in manual assertion	Figure 6-Thin layer chromatography: Shows the 34K protein transfers acyl groups to both water and beta-mercaptoethanol to produce free fatty acid and acyl-S-mercaptoethanol, respectively.	challenge
unacceptable	VIBHA:LUXO	2014-03-23 14:58:56 CDT	GO:0000156 phosphorelay response regulator activity (F)	PMID:10027982	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2: LuxO point mutations at Asp-47 resulting in a non-phosphorylated protein mimic causes constitutive luminescence. LuxO point mutations at Asp-47 resulting in a phosphorylated protein mimic causes nearly no light production.	challenge
requireschanges	VIBCB:LUXO	2014-03-30 17:34:05 CDT	GO:0000156 phosphorelay response regulator activity (F)	PMID:10027982	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2: LuxO point mutations at Asp-47 resulting in a non-phosphorylated protein mimic causes constitutive luminescence. LuxO point mutations at Asp-47 resulting in a phosphorylated protein mimic causes nearly no light production.	challenge
unacceptable	VIBHA:LUXU	2014-04-06 14:59:44 CDT	GO:0000155 phosphorelay sensor kinase activity (F)	PMID:9922254	ECO:0000247 sequence alignment evidence used in manual assertion	Figure 3B - Sequence alignment of known phosphorelay proteins shows the His58 of LuxU is highly conserved	challenge

acceptable:1
unacceptable:2
requires_changes:1
flagged:0

Annotations challenged by Megans

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	John.evenocheck, Team Just Name It Something	SFV:POLS	GO:0016021 - integral component of membrane (C)	PMID:17483865	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
requireschanges	Mnxj39, Team blindmice	HUMAN:NEU1	GO:0090303 - positive regulation of wound healing (P)	PMID:24205344	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3
unacceptable	Dalton.Hassler, Team Awesome 2014	PSYA2:CH10	GO:0006457 - protein folding (P)	PMID:7901771	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
unacceptable	Rachael.Masset, Team A5	STAA8:FNBA	GO:0007155 - cell adhesion (P)	PMID:18375547	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Megans, Team Rubby ducky	PHOPO:LUXC	GO:0050062 - long-chain-fatty-acyl-CoA luciferin component reductase activity (F)	PMID:6833298	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2 fixed by Megans
requireschanges	Kaitlinm, Team Rubby ducky	VIBF1:Q5E6Z3	GO:0000155 - phosphorelay sensor kinase activity (F)	PMID:15916958	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 4

1 annotations fixed by Megans

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