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Category:Team A5

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableSTAA8:ARLSKimberly.heggeset, Team A52014-04-06 14:05:19 CDTGO:0008236 serine-type peptidase activity (F)PMID:10869073ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2

Arls mutant strains showed a decrease in serine protease activity and an increase in autolysin activity when exposed to a serine protease inhibitor

challenge
unacceptableSTAA8:ARLSRachael.Masset, Team A52014-04-06 13:47:17 CDTGO:0000155 phosphorelay sensor kinase activity (F)PMID:10869073ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2.

The mutant showed less extracellular proteolytic activity. Which is responsible for increased autolysis of mutant cell

challenge
updatedbyinstructorHUMAN:FIBBKimberly.heggeset, Team A52014-04-06 13:35:26 CDTGO:0043152 induction of bacterial agglutination (P)PMID:24367264ECO:0000314 direct assay evidence used in manual assertion

Figure 2.

Fg induced agglutination in all of wild type strains of S. aureus

challenge
updatedbyinstructorHUMAN:FIBAKimberly.heggeset, Team A52014-04-06 13:33:59 CDTGO:0043152 induction of bacterial agglutination (P)PMID:24367264ECO:0000314 direct assay evidence used in manual assertion

Figure 2.

Fg induced agglutination in all wild type strains of S. aureus.

challenge
unacceptableSTAA8:ARLRRachael.Masset, Team A52014-04-06 13:14:13 CDTGO:0009405 pathogenesis (P)PMID:11454217ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2

hla production is expressed at much higher levels in the mutants used in the experiment than those of the wildtypes. This information concluded that the arl operon increases the production of virulence factor proteins.

challenge
requireschangesSTAAU:Q9AEP9Rachael.Masset, Team A52014-04-06 12:59:44 CDTGO:0044011 single-species biofilm formation on inanimate substrate (P)PMID:18375547ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4

A mutation in both fnbpA and fnbpB leads to a dramatic decrease in the amount of biofilm that is formed by the bacteria.

challenge
unacceptableSTAAU:C7BUR8Brookelle.christman, Team A52014-04-04 12:41:40 CDTGO:1900233 positive regulation of single-species biofilm formation on inanimate substrate (P)PMID:19851500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8:

When the expression of the sasC gene products were inhibited in an S. aureus strain, biofilm production was reduced in comparison to the S. aureus wild type strain.

challenge
unacceptableSTAA8:ARLSBrookelle.christman, Team A52014-04-04 12:34:16 CDTGO:0009405 pathogenesis (P)PMID:10869073ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2:

The mutant showed less extracellular proteolytic activity, which is responsible for increased autolysis of mutant cells.

challenge
updatedbyinstructorSTAA8:Q2FWJ1Brookelle.christman, Team A52014-04-04 12:23:53 CDTGO:0044011 single-species biofilm formation on inanimate substrate (P)PMID:19188357ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 (C&D):

Sigma factor B is needed for biofilm formation in S aureus.

challenge
updatedbyinstructorSTAAU:C7BUR8Brookelle.christman, Team A52014-03-20 14:49:35 CDTGO:0016020 membrane (C)PMID:19851500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8:

When the expression of the sasC gene products were inhibited in a S. aureus strain, biofilm production was reduced in comparison to the S. aureus wild type strain.

challenge
unacceptableSTAA3:Q2FF57Brookelle.christman, Team A52014-03-20 14:31:09 CDTGO:0003824 catalytic activity (F)PMID:19188357ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 (C&D):

Sigma factor B is needed for biofilm formation in S aureus.

challenge
unacceptableSTAAU:HLABrookelle.christman, Team A52014-03-20 14:16:20 CDTGO:0009405 pathogenesis (P)PMID:11454217ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2:

Hla production is expressed at much higher levels in the mutants used in the experiment than those of the wildtypes. This information concluded that the arl operon increases the production of virulence factor proteins.

challenge
unacceptableSTAA8:ARLSBrookelle.christman, Team A52014-03-20 14:04:14 CDTGO:0008236 serine-type peptidase activity (F)PMID:10869073ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2:

The mutant showed less extracellular proteolytic activity. Which is responsible for increased autolysis of mutant cells.

challenge
unacceptableSTAAE:A6QJY9Brookelle.christman, Team A52014-03-20 13:43:20 CDTGO:0005576 extracellular region (C)PMID:13278487ECO:0000315 mutant phenotype evidence used in manual assertion

Table 5:

Compares the conditions required for absorption and clumping between fibrinogen and antibodies produced by Staphylococcus aureus species

challenge
flaggedSTAAR:FNBABrookelle.christman, Team A52014-03-20 13:17:42 CDTGO:0042710 biofilm formation (P)PMID:18375547ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4 shows fnbpA contributes to glucose induced biofilm development of S. aureus

challenge
acceptableSTAAE:CLFARachael.Masset, Team A52014-03-02 16:36:07 CSTGO:0009405 pathogenesis (P)PMID:22028651ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 1

Clumping Factor A has shown to be the most important of a three gene system: clfA, vwb, and coa. Mutations to clfA severely impaired agglutination.

challenge
requireschangesSTAA3:EBHRachael.Masset, Team A52014-03-02 16:34:30 CSTGO:0030313 cell envelope (C)PMID:12438342ECO:0000314 direct assay evidence used in manual assertion

Fig 4.

When the Ebh protein was removed from the cell wall by boiling it in a buffer there was no lines visible for the Western Blot. Meaning the protein is bound to the cell envelope.

challenge
unacceptableSTAA3:ARLSRachael.Masset, Team A52014-03-02 16:32:54 CSTGO:0000155 phosphorelay sensor kinase activity (F)PMID:10869073ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2.

The mutant showed less extracellular proteolytic activity. Which in turn is responsible for increased autolysis of mutant cells. Could very well be part of the reason S. aureus is virulent.

challenge
unacceptableSTAA4:D3EUQ4Rachael.Masset, Team A52014-03-02 16:31:08 CSTGO:0007155 cell adhesion (

Used in agglutination Processes (Fg) induces agglutination of S. aureus wildtype strains similarly to 2.5% HP. Mutants in arlRS were unable to agglutinate with Fg.

)
PMID:13278487IMP: Inferred from Mutant Phenotype
challenge
unacceptableSTAA8:FNBARachael.Masset, Team A52014-03-02 16:27:52 CSTGO:0007155 cell adhesion (P)PMID:18375547ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4

fnbpA contributes to glucose induced biofilm development of S. aureus.

challenge

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Pages in category "Team A5"

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