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PMID:6147345
| Citation |
Carey, LM, Rodriguez, A and Meighen, E (1984) Generation of fatty acids by an acyl esterase in the bioluminescent system of Photobacterium phosphoreum. J. Biol. Chem. 259:10216-21 |
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| Abstract |
The fatty acid reductase complex from Photobacterium phosphoreum has been discovered to have a long chain ester hydrolase activity associated with the 34K protein component of the complex. This protein has been resolved from the other components (50K and 58K) of the fatty acid reductase complex with a purity of greater than 95% and found to catalyze the transfer of acyl groups from acyl-CoA primarily to thiol acceptors with a low level of transfer to glycerol and water. Addition of the 50K protein of the complex caused a dramatic change in specificity increasing the transfer to oxygen acceptors. The acyl-CoA hydrolase activity increased almost 10-fold, and hence free fatty acids can be generated by the 34K protein when it is present in the fatty acid reductase complex. Hydrolysis of acyl-S-mercaptoethanol and acyl-1-glycerol and the ATP-dependent reduction of the released fatty acids to aldehyde for the luminescent reaction were also demonstrated for the reconstituted fatty acid reductase complex, raising the possibility that the immediate source of fatty acids for this reaction in vivo could be the membrane lipids and/or the fatty acid synthetase system. |
| Links | |
| Keywords |
Aldehyde Oxidoreductases/isolation & purification; Fatty Acids/biosynthesis; Kinetics; Luminescent Measurements; Mercaptoethanol/pharmacology; Molecular Weight; Palmitoyl-CoA Hydrolase/isolation & purification; Palmitoyl-CoA Hydrolase/metabolism; Photobacterium/enzymology; Thiolester Hydrolases/isolation & purification |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0016746: transferase activity, transferring acyl groups |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0016746: transferase activity, transferring acyl groups |
ECO:0000314: |
F |
Figure 6-Thin layer chromatography: Shows the 34K protein transfers acyl groups to both water and beta-mercaptoethanol to produce free fatty acid and acyl-S-mercaptoethanol, respectively. |
complete | ||||
See also
References
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