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Category:MichSt14A 19

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableRHOS4:Q3J2P5Readettd, MichSt14A 192014-04-06 15:50:59 CDTGO:0015250 water channel activity (F)PMID:24497982ECO:0000314 direct assay evidence used in manual assertion

Figure 7 shows the average water permeability of the aquaporin to be 1.23±0.43×10−12 cm3/s

challenge
unacceptableFRATT:Q5NGM8Readettd, MichSt14A 192014-04-06 15:15:40 CDTGO:0016021 integral component of membrane (C)PMID:24593131ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the CapA-frGFP fusion protein, which was used to identify CapA as an integral inner-membrane protein with both the N- and C- termini oriented towards the cytoplasm.

challenge
updatedbyinstructor9ROSI:C0LU17Readettd, MichSt14A 192014-04-06 13:39:44 CDTGO:0036263 L-DOPA monooxygenase activity (F)PMID:24613318ECO:0000314 direct assay evidence used in manual assertion

Table 3 shows a diphenolase activity of kcat 199.3 s^-1 towards L-dopa

challenge
acceptable9ROSI:C0LU17Readettd, MichSt14A 192014-04-06 13:36:34 CDTGO:0004503 monophenol monooxygenase activity (F)PMID:24613318ECO:0000314 direct assay evidence used in manual assertion

Table 3 shows a monophenolase activity of kcat 20.8 s^-1 towards L-tyrosine

challenge
acceptableYEAST:AGP1Readettd, MichSt14A 192014-04-06 12:12:42 CDTGO:0015192 L-phenylalanine transmembrane transporter activity (F)PMID:24701347ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows AGP1 has low affinity phenylalanine permease activity

challenge
acceptableYEAST:BAP2Readettd, MichSt14A 192014-04-06 12:02:26 CDTGO:0015192 L-phenylalanine transmembrane transporter activity (F)PMID:24701347ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows BAP2 to be the major permease responsible for high affinity phenylalanine transport

challenge
acceptableYEAST:GAP1Readettd, MichSt14A 192014-04-06 11:50:50 CDTGO:0015192 L-phenylalanine transmembrane transporter activity (F)PMID:24701347ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows phenylalanine transport in wild type cells grown on medium with proline as the sole nitrogen source is catalyzed by Gap1. Gap1 activity is absent when ammonium ion is used as a nitrogen source.

challenge

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