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User:Shasan

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableLAMBD:GT2016-04-20 09:15:49 CDTGO:0098003 viral tail assembly (P)PMID:23911548ECO:0000314 direct assay evidence used in manual assertion

The paper shows through Figure 1 and Figure 4 that gpG and gpGT combine to together to chaperone the tail assembly

challenge
updatedbyinstructorBPSP1:DUHM2016-04-20 09:23:58 CDTGO:0016742 hydroxymethyl-, formyl- and related transferase activity (F)PMID:8527927ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows that deoxyuridylate hydroxymethylase catalyzes the same reaction as L. Casei TS using reactants such as CH2H4 folate and dUMP as well as other reactants in order to yield hmdUMP and H4 Folate.

challenge
unacceptableLAMBD:STF2016-04-20 09:26:50 CDTGO:0019062 virion attachment to host cell (P)PMID:10629200ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows that the when the Tail Fiber Protein in Phage Lambda was inhibited, binding to LamB, the receptor at the surface of Escherichia coli K-12, was greatly reduced.

challenge
unacceptableLAMBD:FI2016-04-21 16:04:14 CDTGO:0019072 viral genome packaging (P)PMID:9220002ECO:0000315 mutant phenotype evidence used in manual assertion

Lambda FI mutants were found to be unable to package DNA efficiently, and the cell had to instead use high concentrations of terminase in order to package the DNA as shown by Figure 7.

challenge
acceptableBPT4:DMA2016-04-25 09:42:36 CDTGO:0032259 methylation (P)PMID:2510127ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 shows that the mutant phenotype of T4 with a different amino acid at residue 126 had higher methylation rates of different amino acids, due to relaxation in sequence specificity caused by the mutation.

challenge
acceptableBPT4:DPOL2016-04-25 15:15:47 CDTGO:0008408 3'-5' exonuclease activity (F)PMID:15037066ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 and Figure 5 show that the mutant phenotype of T4 that is lacking exonuclease has altered DNA polymerase activity. The wild type T4 used the proof reading exonuclease to stop at lesions in the DNA, whereas the mutant phentotype DNA polymerase bypassed the lesions due to lack of exonuclease.

challenge
updatedbyinstructorLAMBD:TIPJ2016-05-04 15:45:33 CDTGO:0046813 receptor-mediated virion attachment to host cell (P)PMID:10629200ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 shows that the when the Tail Fiber Protein in Phage Lambda was inhibited, binding to LamB, the receptor at the surface of Escherichia coli K-12, was greatly reduced.

challenge

acceptable:2
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by Shasan

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableHeatheca,
Team ContaminatorZ
9CAUD:A0A024B0X2GO:0098020 - obsolete virus tail, minor subunit (C)PMID:22923442ECO:0000314 direct assay evidence used in manual assertionchallengeC: 5
unacceptableHoang5,
Team Purple A
9CAUD:A0A0K2CZX3GO:0009186 - deoxyribonucleoside diphosphate metabolic process (P)PMID:11875520ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
unacceptableMnguy1,
Team Blue B
9BACI:A0A0J8FEV2GO:0016301 - kinase activity (BlastP result showed sequence alignment had a 41% identity and an e value of 5E-58.)PMID:11292795ISS: Inferred from Sequence Similarity UniProtKB:D5NZZ3 challengeC: 2
unacceptableMnguy1,
Team Blue B
9CAUD:A0A0K2CZZ1GO:0004604 - phosphoadenylyl-sulfate reductase (thioredoxin) activity (F)PMID:7588765ECO:0000250 sequence similarity evidence used in manual assertionchallengeC: 6
unacceptableJamesMcDermott,
Team T7
BPT4:GLRXGO:0015035 - protein-disulfide reductase activity (F)PMID:3735493ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
unacceptableRacheleBonasera,
Team T1
BPPH2:SSBGO:0003697 - single-stranded DNA binding (F)PMID:6809534ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3

0 annotations fixed by Shasan

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