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User:RacheleBonasera

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorBPPH2:CAPSD2016-04-23 16:00:34 CDTGO:0039617 T=3 icosahedral viral capsid (C)PMID:11562162ECO:0000314 direct assay evidence used in manual assertion

Fig 3

challenge
unacceptableBPPH2:SSB2016-04-23 16:23:16 CDTGO:0003697 single-stranded DNA binding (F)PMID:6809534ECO:0000315 mutant phenotype evidence used in manual assertion
challenge
unacceptableBPP4:VEPS2016-05-01 21:03:16 CDTGO:0048525 negative regulation of viral process (P)PMID:9151842ECO:0000315 mutant phenotype evidence used in manual assertion

As shown in figure 8, there is a failure in derepression activity after the induction of the epsilon am104 mutant

challenge
unacceptableBPP4:RCII2016-05-02 22:11:22 CDTGO:0019076 viral release from host cell (P)PMID:2403440ECO:0000315 mutant phenotype evidence used in manual assertion

Mutants of cII are unable to produce protein C (figure 3a) which regularly causes efficient killing of e.coli.

challenge
updatedbyinstructorBPP4:VPSU2016-05-02 22:59:02 CDTGO:1990216 positive regulation by symbiont of host transcription (P)PMID:1938879ECO:0000314 direct assay evidence used in manual assertion

psu suppresses rho dependent polarity by antitermination. Figs 3,5 show increased expression of beta galactoside in psu+ cells

challenge
acceptableBPP2:BPW2016-05-03 13:06:38 CDTGO:0098025 virus tail, baseplate (C)PMID:24097944ECO:0000314 direct assay evidence used in manual assertion

Increased area of density in wt, illustrate where gpW is localized in the baseplate as seen in Fig 1.

challenge
unacceptableBPP2:VPR2016-05-03 19:25:29 CDTGO:0098003 viral tail assembly (P)PMID:8178426ECO:0000314 direct assay evidence used in manual assertion

Protein R is identified by preparing cultures that were induced or uninduced, running SDS and comparing plaque formation (Figure 3)

challenge
unacceptableBPP2:VPS2016-05-03 19:30:31 CDTGO:0098003 viral tail assembly (P)PMID:8178426ECO:0000314 direct assay evidence used in manual assertion

Protein not present in uninduced cells (fig4). function of protein was verified by plate complementation experiments

challenge

acceptable:1
unacceptable:5
requires_changes:0
flagged:0

Annotations challenged by RacheleBonasera

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by RacheleBonasera

What do the icons mean in the status column?

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